FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length49-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATGAAACGAATTGCTGTTCCTTCATTTTAATGCACAGTTTTTTTTAGA13.125No Hit
ACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGAGGCTTA13.125No Hit
CTATGATGGACCATGTAACGAACAATGCTACAGGGATGAATATTATGGAG13.125No Hit
CTCATCAACAACCGACTAATCACCACCCAACAATGACTAATCAAACTAAC13.125No Hit
CGTATATACGTACAGACGTATTTATTTATATACTATGTCTCTTATACATA13.125No Hit
TATCACAACACAAGAACACCTCTGATTACTCCTGCCATCATGACCCTTGG13.125No Hit
GACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACAGTCCCACCC13.125No Hit
ACAGGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTAT13.125No Hit
GGGTAGAGGGGGCGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT13.125No Hit
GTTTGCGGTTTCGATGATGTGGACTTTGGAGTAGGAACCTGTGAGGGAAG13.125No Hit
GGCCTATGATTGACTACAAAAAGGATTAGACTGAGCCGAATTGGTATATA13.125No Hit
AAACCACACTTATCCCCACCTTGGCTATCATCACCCGATGAGGCAACCAG13.125No Hit
GTGACGTCTTATTTAAGGGGAACGGGTGGGCTATTTAGGCTTTATGACCC13.125No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC13.125No Hit
CACATCAACTGCAACTCCAAAGCCACCCCTCACCCACTAGGATATCAACA13.125No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT13.125No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC13.125No Hit
ATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAA13.125No Hit
ATGTCTCCATCTATTGATGAGGGTCTTACTCTTTTAGTATAAATAGTAC13.125No Hit
GCAAGCCTCACAGTACTGCGGGGCTGCCTTCACCAGTTAGGACGGCATCT13.125No Hit
AGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGA13.125No Hit
GTAATGATGTCGGGGTTGAGGGATCGGAGGTGCATGGGGGATAGGTGTAT13.125No Hit
CAGCGCTCATATAGTGGGGTCGTGTCGAAGAGAGGAGATCTACTTTGCGG13.125No Hit
GATTAACCTTTAGCAATAAACTAAAGTTTAATTAAGCTATACTAACCCCA13.125No Hit
ATATAGAGGAGACAAGTCGTAACATGGTAAGTGTACTGGAAAGTGCACTT13.125No Hit
TACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGT13.125No Hit
CGTCTTTACGTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTG13.125No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAG13.125No Hit
TCCCCACCCCATCCAACATCTCCGCATGGTGAAACTTCGGCTCACTCCTT13.125No Hit
CCCTACTAATCGGTGCCCCCGATATGGCGTTCCCCCGCATACACAACATA13.125No Hit
GGTCTAGGTCTCTGATAAGGATAATGATTGAGAGTGCGAGGTAATGAATG13.125No Hit
CACCATGTCTGTCCTCAAAAAGTTTCAGCTTTTGGATTTGGGATGCTCAA13.125No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)