FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97891
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1520.1552747443585212No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1460.14914547813384274No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1320.1348438569429263No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1140.11645605826889091No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1110.11339142515655166No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1110.11339142515655166No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAGAGTCATGTCA1070.10930524767343269No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1070.10930524767343269No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1040.10624061456109346No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1040.10624061456109346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGGG609.531504E-1082.984173
CCCTGGG609.531504E-1082.984172
GGCTATA300.002082338671.129291
TTGCGAG451.3062087E-463.2260369
GGGCTTA350.003836232260.9679576
GTGGGAC350.003836232260.9679579
GGCGCTA350.003836232260.9679578
GCGCTAT350.003836232260.9679579
CGAATCA752.2357497E-456.685745145
GCATTGC553.5212497E-451.730396
GATAGCA553.5212497E-451.730392
GTCATAA650.00905325449.05497145
ATAGCAT605.408875E-447.4195253
TCTAGCA752.8361632E-547.4195253
CTCTAGC1101.1429074E-745.264092
ACCCTGG952.1702726E-644.923761
CCACAGG1452.4988021E-643.98032145
TAGGCGC658.023894E-443.771876
CAGCGCA1054.3197706E-640.6453139
CATGCAG700.001155518140.6453065
Produced by FastQC (version 0.11.5)