FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81858
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1350.16491973906032398No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1240.15148183439614943No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1130.13804392973197488No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1110.1356006743384886No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1040.1270492804612866No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT1010.12338439737105719No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC940.11483300349385522No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT930.11361137579711207No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC900.10994649270688266No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC890.1087248650101395No Hit
CTCTAACACTATGCTTAGGCGCTATCACCACTCTGTTCGCAGCAGTCTGC870.10628160961665323No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT870.10628160961665323No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN860.10505998191991009No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA830.10139509882968066No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC830.10139509882968066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)