FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72501
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA1660.22896235913987392No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1360.18758361953628225No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1240.1710321236948456No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1230.16965283237472586No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1140.15723921049364836No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1140.15723921049364836No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1110.1531013365332892No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT1020.1406877146522117No Hit
CATTCATACAGGTCCCTATTTAAGGAACAAGTGATTATGCTACCTTTGCA1020.1406877146522117No Hit
ACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCGTTTATTGCTAAAGGTT990.13654984069185253No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGGGTGATAT970.13379125805161307No Hit
GGCCTAGCCCTACTAGTCTCAATCTCCAACACATATGGCCTAGACTACGT940.1296533840912539No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC930.1282740927711342No Hit
TCCTAACCCTACTCCTAATCACATAACCTATTCCCCCGAGCAATCTCAAT850.11723976221017642No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA820.11310188824981725No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC810.11172259692969752No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC800.1103433056095778No Hit
CTCTAGAGGAGCCTGTTCTATAATCGATAAACCCCGATCAACCTCACCAC790.10896401428945807No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG780.10758472296933834No Hit
ACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACCAC770.10620543164921864No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG760.10482614032909891No Hit
AGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACTAGTCTCAA750.10344684900897919No Hit
GTACAAGGAAGGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGT740.10206755768885946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG100.0074187797142.733111
TAAGACT100.0074187797142.733119
CGCATGA401.0061298E-5105.9176145
CTAGATA259.955454E-485.639864
CCTAGAT259.955454E-485.639863
GGCCGTA259.955454E-485.639866
TGGCCGT259.955454E-485.639865
CCGTACG259.955454E-485.639868
GCCCTGG259.955454E-485.639861
CGTACGC259.955454E-485.639869
CTGGCCG259.955454E-485.639864
GCCGTAC259.955454E-485.639867
GAAATCG400.001339203379.4382145
CATCTCT300.00205287171.3665549
GTCTACG300.00205287171.3665542
TACGGAG300.00205287171.3665545
CTACGGA300.00205287171.3665544
GACTATC350.003153045464.04219135-139
GGTCTAC451.2826393E-463.4369351
CCTGGCC350.003782030461.171333
Produced by FastQC (version 0.11.5)