FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50489
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCA950.18815979718354495No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC910.18023727940739567No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT760.15052783774683595No Hit
CTCCTAGCCTTACTACTAATAATCATTACATTTTGACTACCACAACTCAA630.12477965497435085No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA630.12477965497435085No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT620.12279902553031354No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA600.11883776664223891No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC590.1168571371982016No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT560.11091524886608964No Hit
GTCTAGGCCATATGTGTTGGAGATTGAGACTAGTAGGGCTAGGCCCACCG520.10299273108994038No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAATAGGGA510.10101210164590306No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC510.10101210164590306No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA510.10101210164590306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)