FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139716
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC2250.16104096882246843No Hit
GACTACAACCACGACCAATGATATGAAAAACCATCGTTGTATTTCAACTA2100.15030490423430387No Hit
GTTGTAGTCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT1700.12167539866586505No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1630.11666523519138824No Hit
GTGGGTGGTTGTGTTGATTCAAATTATGTGCTTTTTGGAAAGTCATGTCA1610.1152337599129663No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC1600.11451802227375535No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT1600.11451802227375535No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAA1570.11237080935612243No Hit
GGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAAAACTAATC1570.11237080935612243No Hit
GGCTAGGAGGGTGTTGATTATTAAAATTAAGGCGAAGTTTATTACTCTTT1530.10950785879927853No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1510.10807638352085659No Hit
GCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATCTTCTTACTCAT1480.10592917060322368No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC1450.10378195768559076No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT1430.10235048240716882No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG1420.10163474476795786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAG451.3794033E-678.988419
CCGTGCG851.5115802E-858.5443579
GGTATAC502.2085856E-456.871663
CCAGAAA600.00564413155.298496145
TGCAGCT553.5362362E-451.7015047
CCTTCTG701.8965335E-550.7782639
ATTAGAG1550.050.4506653
CATGTAC851.0156036E-650.1808783
CAATGCA700.001160409347.398712145
GAGGCAC1650.047.393057
GTATAAC605.431852E-447.3930473
GCAGCTC605.431852E-447.3930478
GCATGTA901.5060104E-647.3930472
GCTGTGT1203.3563178E-546.08208145
CGCATGA1502.4101482E-644.238792145
GACTGAG658.0579176E-443.747437
TAGTACT658.0579176E-443.747434
GTAGCAC700.001160409340.6226123
TAGTCAA2201.8060746E-840.217087145
ATTAGAC1253.1459422E-739.810163
Produced by FastQC (version 0.11.5)