FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348290
Sequences flagged as poor quality0
Sequence length20-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAAATTTC4179612.000344540469149No Hit
AAAACCAACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAAATTTC3649510.47833701800224No Hit
ATACATCCAAACCTATTAAATTCTCTCTCCAACACACCCACTTCTAACAA256037.351058026357346No Hit
ATACGTCCAAACCTATTAAATTCTCTCTCCAACACACCCACTTCTAACAA244757.02718998535703No Hit
AAAAAACAACCCAACCTAAACAAACAAACAAAACCAAACAAAAAACAAAT102732.949553532975394No Hit
AAAAAACAACCCGACCTAAACAAACAAACAAAACCAAACAAAAAACAAAT91432.6251112578598295No Hit
CCGCCCCTTTCTCCATAAATAAAAAAAAAACCCCTATTTAAAAAAATCTC83482.396853197048437No Hit
CCACCCCTTTCTCCATAAATAAAAAAAAAACCCCTATTTAAAAAAATCTC81062.3273708691033335No Hit
GTCTCCTTCCACCCACAAAATCCATCCCCAACAAACAAACAATAACTCAA58241.6721697436044676No Hit
ATCTCCTTCCACCCACAAAATCCATCCCCAACAAACAAACAATAACTCAA52461.506216084297568No Hit
TCCGAAATCAAATTCCTCCCCCTCAACCCAACCAAAAAACACCAACAAAA48391.38935944184444No Hit
ATAAAACAAAATACAAACAACTTTAAAATACCAACATTCAACTCCCTACA47431.3617962043124983No Hit
CCCGAAACTAATAAAAATTTATAAACTCCCACAACAACCCCCAAATTATC45801.314996123919722No Hit
AAAAACCACTTAAAAACCTCAAACACAAATTAACAACAAACACTAAAAAA44781.285710184042034No Hit
TCCAAAATCAAATTCCTCCCCCTCAACCCAACCAAAAAACACCAACAAAA44641.2816905452352925No Hit
TAACACGCCCTCTAAAAAAACTTCAAAAAAAAAACAAAATAACAAAAATC40681.1679921904160326No Hit
CCGCCCCTTTCTCCATAAATAAAAAAAAACCCCTATTTAAAAAAATCTCC38461.1042522036234173No Hit
CCACCCCTTTCTCCATAAATAAAAAAAAACCCCTATTTAAAAAAATCTCC37471.0758276149186023No Hit
AACTTTCAACAAACCTCTTATCCAAAAACAATCTACAAAAACCACCTCCA37201.0680754543627438No Hit
CTATTAATAACGCTTAACAACTACAAAAACACCCACAATCCCTACCCCCA36711.0540067185391484No Hit
CCCAAAACTAATAAAAATTTATAAACTCCCACAACAACCCCCAAATTATC36141.037641046254558No Hit
CTATTAATAACACTTAACAACTACAAAAACACCCACAATCCCTACCCCCA35031.0057710528582504No Hit
CCCCCTACTCTAACATCTTAAAAACAATTAAAAAAACACATCCCAAAAAC28710.8243130724396336No Hit
TAACACACCCTCTAAAAAAACTTCAAAAAAAAAACAAAATAACAAAAATC26440.7591375003588964No Hit
AAACTCGCTCTACCCCTTAACAACAAACACCACCAATACCCCTACAAATA25390.728990209308335No Hit
TAACACGCCCTCTAAAAAAACTTCAAAAAAAAACAAAATAACAAAAATCA23450.6732895001292026No Hit
AAACTCACTCTACCCCTTAACAACAAACACCACCAATACCCCTACAAATA23450.6732895001292026No Hit
TACCGACAAATCCCCCACCACCACATCATCTAAACAACCAAATCTTCAAC17760.5099198943409228No Hit
TACCAACAAATCCCCCACCACCACATCATCTAAACAACCAAATCTTCAAC15900.4565161216227856No Hit
TAACACACCCTCTAAAAAAACTTCAAAAAAAAACAAAATAACAAAAATCA14680.4214878405926096No Hit
CTATTAATAACGCTTAACAACTACAAAAACACCCACAATCCCTACCCCCC12260.35200551264750635No Hit
NAAACCGACCTANTCTCTCTAAAACAACATTANAAAAACCNCCAAATNTC11900.34166929857302825No Hit
CTATTAATAACACTTAACAACTACAAAAACACCCACAATCCCTACCCCCC11830.3396594791696575No Hit
ACGCCCTCCTCAAACCAAACACCACCAAAACCTCCCCAAAACCCAAACTC10710.3075023687157254No Hit
NAAACCAACCTANTCTCTCTAAAACAACATTANAAAAACCNCCAAATNTC10170.29199804760400816No Hit
NTACATCCAAACNTATTAAATTCTCTCTCCAANACACCCANTTCTAANAA7180.2061500473743145No Hit
NTACGTCCAAACNTATTAAATTCTCTCTCCAANACACCCANTTCTAANAA6900.1981107697608315No Hit
ACACCCTCCTCAAACCAAACACCACCAAAACCTCCCCAAAACCCAAACTC6580.1889230239168509No Hit
CCGCCCCTTTCTCCATAAATAAAAAAAAAAACCCCTATTTAAAAAAATCT5890.16911194694076775No Hit
ACGACAAACTTAAAAACAAATTACAAACAAAATAATAAATTACTAACAAA5700.16365672284590427No Hit
CCACCCCTTTCTCCATAAATAAAAAAAAAAACCCCTATTTAAAAAAATCT5630.16164690344253352No Hit
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAACTTTC5100.14642969938844067No Hit
NAAAAACAACCCNACCTAAACAAACAAACAAANCCAAACANAAAACANAT4970.14269717763932355No Hit
ACAACAAACTTAAAAACAAATTACAAACAAAATAATAAATTACTAACAAA4610.1323609635648454No Hit
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAACCACCAAATTTCT4430.1271928565276063No Hit
AAAACCAACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAACTTTC4420.1269057394699819No Hit
AAAACCGACCTACTCTCTCTAAAACAACATTAAAAAAACCACCAAATTTC3820.1096787160125183No Hit
ATACATCCAAACCTATTAAATTCTCTCTCCAACACGCCCACTTCTAACAA3690.10594619426340118No Hit
ATAAAACAAAATACAAACAACTTTAAAATACCAACATTCAACCCCCTACA3590.10307502368715725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)