FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363146
Sequences flagged as poor quality0
Sequence length20-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG336599.268723874144284No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG333929.195199726831632No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG315768.695125376570305No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG286567.891041068881386No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG202925.5878351957614845No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG160034.406767526008823No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG136953.771210477328678No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG135423.7290786625764842No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG130923.6051615603641514No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG114353.148871252884515No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG69561.9154830288644238No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT65641.8075374642705688No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT57701.5888926217003627No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG49911.3743783492039015No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT46571.282404322228525No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG46151.2708387260220408No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT42911.1816184124291607No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT34830.95911837112346No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG34550.9514079736524704No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT33090.9112037582680245No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG29110.8016059656446719No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG25500.7021969125365555No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG19030.5240316566890452No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT18240.5022773209673245No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT17800.4901609820843407No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT17740.48850875405484295No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT15380.42352111822793037No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA14190.39075192897622446No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT13840.3811139321374874No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA13200.3634901664895111No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT12810.35275068429777556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11220.30896664151608444No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG10240.2819802503676207No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG9780.26931316880813777No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG9750.2684870547933889No Hit
GTTTTTGAGTGGGTTTGGAGTCGTGGAGTTGGGTGGGGGTGGGAAGGAGG9500.26160277133714815No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG9280.25554460189565625No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGCAG9100.25058791780716294No Hit
GTTTTCGAGTGGGTTTGGAGTCGTGGAGTTGGGTGGGGGTGGGAAGGAGG8930.24590660505691928No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTCGTTAAGGGGTAGAGTG8920.2456312337186696No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT8890.24480511970392077No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG8590.23654397955643183No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT8440.2324134094826874No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGCAG7600.2092822170697185No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA6570.18091896923000667No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG5960.16412131759677925No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTCGTTAAGGGGTAGAGTG5920.16301983224378075No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA5800.15971537618478518No Hit
TTGGGGGTGGAGTGGGAGCGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG5770.1588892621700363No Hit
TCGGGGGTGGAGTGGGAGCGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG5450.15007737934604815No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT5170.1423669818750585No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGCTGAAGTAG4930.13575806975706742No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG4580.12612007291833036No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGCGTGAGGGTGTATATGTGGG4420.1217141315063363No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG4410.12143876016808668No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG4380.12061264615333778No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG4320.11896041812384No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGCGTGAGGGTGTATATGTGGG4030.11097464931460073No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG3840.10574259388785778No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGCTGAAGTAG3770.10381499452011038No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTATATTAGATAATGT3760.10353962318186073No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT3720.10243813782886221No Hit
ACGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG3640.10023516712286519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)