FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319064
Sequences flagged as poor quality0
Sequence length20-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG302949.494646842012887No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG286188.969360379108894No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG277618.70076222952135No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG248977.803136674773713No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG167495.249417044856204No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG151924.76142717448537No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG125123.9214703006293408No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG117823.692676077526766No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG112333.5206102850838703No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG101173.1708371988065087No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG61651.9322142266128426No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT60481.8955444675676354No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT55051.7253591755885966No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG48001.5044003710854248No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG45391.422598600907655No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT41731.3078880726123912No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT38081.1934909610611038No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT33001.0342752551212298No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT30400.9527869016874357No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG28940.9070280570669208No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG24790.7769601083168267No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG23660.7415440162475241No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT19590.613983401449239No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT19010.5958052302986234No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT18120.5679111400847479No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG16690.5230925456961613No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA15150.4748263671238372No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA14080.44129077551839124No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAGGGGGTAGAGTG12110.3795476769550936No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT11880.3723390918436426No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT11350.35572800441290775No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT10790.3381766667502445No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10430.3268936639671038No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT10270.3218789960634857No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG9970.3124764937442018No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG8940.28019456911466034No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT8500.26640423237971067No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG8410.2635834816839255No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG7960.2494797282049996No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG7450.23349547426221698No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAGGGGGTAGAGTG7320.22942105659052728No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA7050.22095880450317176No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA7000.2193917207832911No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG5320.16673770779530125No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT4630.14511195246094827No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG4440.1391570343254018No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT4290.13445578316575985No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTATATTAGATAATGT3820.11972519619888172No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT3770.11815811247900107No Hit
ACGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG3740.11721786224707269No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTCTTTTAGTGTGAGAGTGTGGTG3470.10875561015971719No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG3410.10687510969586038No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG3280.10280069202417069No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG3210.10060677481633779No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATA1900.0135.964755
TAAGCGT12100.0135.964755
AAGCGTT12100.0135.964756
TGTATAA1900.0135.964756
GTCATTC100.008591883135.964744
CCCAGAG100.008591883135.964746
GCCCAGA100.008591883135.964745
TCAGGGA151.5882602E-4135.964749
GATGCCC100.008591883135.964742
TTCGAGC255.4407792E-8135.964744
CCAGAGA100.008591883135.964747
CATTCGT100.008591883135.964746
TTATACA100.008591883135.964749
TGCCCAG100.008591883135.964744
TCTAAGT100.008591883135.964749
GTGGGCT100.008591883135.964749
GGCTTGA100.008591883135.964747
AGAGACT100.008591883135.964747
AGATGCC100.008591883135.964741
CGAGCGG255.4407792E-8135.964746
Produced by FastQC (version 0.11.5)