FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313038
Sequences flagged as poor quality0
Sequence length20-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG258338.25235274950645No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG252848.07697468039024No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG234607.494297816878462No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG215346.879037049815039No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG160815.137076009941285No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG146534.6809013602182485No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG121093.8682204716360316No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG120383.8455395191638075No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG102783.2833074578805124No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG101093.229320401995924No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG80772.5801979312415746No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT70112.2396641941233972No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT64842.071314025773229No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG54631.7451555402219538No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG50181.60300027472703No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT40911.3068700924488401No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT37611.2014515809582225No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG34811.1120055712086072No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG32641.0426849136526557No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT31441.004350909474249No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG29920.955794504181601No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT28640.9149048997246341No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT25600.8177920891393378No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT24410.7797775349957513No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT22150.7075818271264191No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG19780.6318721688740664No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA15170.4846057028220216No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA14630.46735540094173866No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT12600.4025070438732678No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT12390.3957985931420467No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT11790.37663159105284344No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT11310.36129798938148083No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10890.3478810879190386No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT10680.34117263718781743No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA9330.2980468824871102No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG9180.2932551319648094No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA8640.2760048300845265No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG7770.2482126770551818No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG7490.23926807608022027No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG6860.2191427238865569No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT6790.21690657364281654No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG6220.19869792165807346No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGCATATGTGGG5180.16547511803678788No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGCATATGTGGG4950.15812776723592661No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG4540.14503031580830442No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT4450.14215526549492394No Hit
ATTTGTGTTAAGGGATAGGAGAAGTGGAGTTTGTTTTTTGGGTTTAGAAG4430.14151636542528384No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG4250.13576626479852286No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT4180.13353011455478248No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTATATTAGATAATGT4120.13161341434586216No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG3700.11819651288341991No Hit
CGTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTTG3650.11659926270931964No Hit
ACGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG3570.11404366243075921No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG3570.11404366243075921No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGCTGTTAAGGGGTAGAGTG3540.11308531232629904No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG3430.10957136194327845No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG3380.10797411176917818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)