FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302188
Sequences flagged as poor quality0
Sequence length20-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG297889.857439739499913No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG287009.497398970177505No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG258028.538393318066898No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG232717.700835241637656No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG157885.224562193071862No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG140804.65935113240764No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG115663.82741869299906No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG113783.7652057659470266No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG102023.3760440520470696No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG99643.297285133757793No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG56201.859769415066118No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT54761.8121169603028577No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT50311.6648576382913949No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG44551.474247819238355No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG40261.3322832144228096No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT38701.2806597217626112No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT34041.1264510834315062No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT31561.0443829668947806No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT29030.9606602512343309No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG27460.9087058387493877No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG23290.7707122718307808No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG21330.7058519861807881No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT16230.5370828755609092No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG16070.5317881583649913No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT15030.4973724965915258No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT14520.4804955855295379No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA13340.4414470462096443No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA12010.3974347095185778No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT11400.3772486002091413No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTGAGGGGTAGAGTG10330.3418401789614412No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT9780.32363958860047387No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT9760.32297774895098413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9440.3123883145591486No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT8950.29617324314665044No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG8890.29418772419818123No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG8680.28723840787853916No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG8160.27003057699180644No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG7920.26208850119792976No Hit
TAAGATGTTAGAGTAGGGGGAGTCCAAGTCTCTGCTACCGTATAAGGTAGGAGGTTTGGTT7080.23429123591936143No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTGAGGGGTAGAGTG6670.22072352310482216No Hit
TAAGATGTTAGAGTAGGGGGAGTCCAAGTCTCTGCTACCGTATAAGGTAGGAGGTTCGGTT6640.2197307636305876No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG6530.2160906455583941No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA6340.20980316888824174No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT6250.206824890465538No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA6030.19954465432115107No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG5160.17075462956834817No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG4010.1326988497226892No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTT3890.12872781182575085No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT3820.12641137305253683No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG3750.12409493427932282No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT3700.1224403351555985No Hit
AACGTCGTTTTAGAGAGATTAGGTCGGTTT3680.1217784955061088No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTATATTAGATAATGT3490.11549101883595642No Hit
ACGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG3370.11151998093901809No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG3220.10655618356784519No Hit
TTGTTAAGCGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG3170.10490158444412087No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT3140.10390882496988631No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGCGTGTGAGGGTGTATATGTGGG3110.1029160654956517No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG3080.10192330602141714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGGA6100.0149.05453140-144
TATATGA203.0527608E-6134.859349
GTCGTCT100.00880387134.859344
GGTCTGA151.6406555E-4134.859347
ATGGCTT100.00880387134.859341
TTCGAGC100.00880387134.859344
GTCGATT100.00880387134.859344
GATAAGA3250.0134.859342
CTAAGGT100.00880387134.859349
GTCTGAG151.6406555E-4134.859348
TTATACA100.00880387134.859349
GGATAAG3250.0134.859341
GTGGGTC100.00880387134.859349
TCTAAGG100.00880387134.859348
ATTAATT203.0527608E-6134.859341
TCGTCTA100.00880387134.859345
TCTGAGG151.6406555E-4134.859349
TCGTTAA100.00880387134.859341
GCGTTCG100.00880387134.859341
TCGTATA1100.0134.859345
Produced by FastQC (version 0.11.5)