FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210715
Sequences flagged as poor quality0
Sequence length21-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG191039.065799776949907No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG175628.334480222100941No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG163597.763566903163039No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG145896.923569750611016No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG114635.440049355764896No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG93084.4173409581662435No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG85484.0566642146975775No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG79053.7515127067365874No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG73663.4957169636713097No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG70013.322497211873858No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG49642.3557886244453408No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT44572.1151792705787438No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT39651.8816885366490284No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG33541.591723417886719No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG30471.4460289965118762No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT27091.2856227606008115No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT25591.2144365612319958No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT22081.0478608547089672No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG20830.9885390219016207No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGCTGGGTGGGGGTGGGAAGGAGG19920.9453527276178726No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT19130.9078613292836296No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGCTGGGTGGGGGTGGGAAGGAGG17950.8518615191134945No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG17700.8399971525520253No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG14990.7113874190256982No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT11600.5505066084521748No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT11400.5410151152029993No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG11390.5405405405405406No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT10770.5111169114680968No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA9260.4394561374368222No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA8210.38962579787865126No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT8050.38203260327931093No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT7740.367320788743089No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6740.3198633224972119No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT6500.3084735305982014No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG5840.27715160287592244No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT5780.2743041549011698No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG5600.265761810976912No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG5310.2519991457656076No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT4930.23396530859217424No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG4780.22684668865529267No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA4410.20928742614431817No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA4240.20121965688251905No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG3890.18460954369646204No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG3560.16894857983532258No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGCG3130.14854186934959543No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTACATGTGGG3110.14759272002467788No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTACATGTGGG2990.14189782407517262No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT2870.13620292812566737No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT2860.1357283534632086No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT2660.1262368602140332No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG2530.12006738960206914No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGCGGG2530.12006738960206914No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG2460.11674536696485774No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTCGGAGTGGTTGAAGTAG2420.11484706831502267No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG2340.1110504710153525No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTCGGAGTGGTTGAAGTAG2300.1091521723655174No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTATATTAGATAATGT2280.10820302304059987No Hit
ACGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG2230.105830149728306No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGCGGG2180.10345727641601216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG2150.0136.283581
TTGTATA1200.0136.283585
ACGTTGT3250.0136.283582
ATTAATT151.572762E-4136.283581
GTTATTC8400.0136.283584
CGTTTTA4200.0136.283586
ATTAGTA100.008529901136.283577
ACGTCGT2050.0136.283572
GATAAGA1600.0136.283572
GGATAAG1600.0136.283571
ATTATAA202.8952363E-6136.283577
TAGTAGG100.008529901136.283579
AAGATGT100.008529901136.283572
CTGGATT100.008529901136.283571
CGGGAGT4350.0136.283572
GGCTTGA100.008529901136.283577
GCGTTTC100.008529901136.283574
GATTATA202.8952363E-6136.283576
TTGCGGG100.008529901136.283578
CGAGAGG100.008529901136.283576
Produced by FastQC (version 0.11.5)