FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386539
Sequences flagged as poor quality0
Sequence length20-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG386399.996145279001603No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG354319.166216086863162No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG354239.164146438004963No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG316908.19839653954711No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG212795.505007256706309No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG185294.793565461699854No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG153003.9582034413086387No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG151363.915775639715527No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG138983.5954974789089844No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG131473.4012091923454038No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG77802.012733514600079No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT65171.6859877011116602No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT63421.6407141323385221No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG57431.4857491740807525No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG53071.3729533113088201No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT43661.129510864362975No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT39751.028356776418421No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG32750.8472625013258689No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT29230.7561979515650425No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT28660.7414517034503634No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG27490.7111830888991797No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG24230.6268448979275054No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT19380.5013724358990943No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT19160.49568090153904265No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG18410.47627794349341207No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA15910.41160141667464345No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT15400.3984074052036146No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA14160.3663278479015054No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT14120.3652930234724051No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT12740.32959158066844485No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT12210.31588015698286587No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG12150.3143279203392154No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11950.30915379819371397No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT11800.30527320658458784No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG10490.2713827065315531No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG10330.2672434088151519No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG9610.24861656909134655No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG9250.23930314922944387No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT8930.23102455379664147No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG6510.16841767583607345No Hit
TAAGATGTTAGAGTAGGGGGAGTCCAAGTCTCTGCTACCGTATAAGGTAGGAGGTTCGGTT5970.15444754604321945No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG5810.15030824832681827No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA5720.14797989336134257No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA5700.14746248114679242No Hit
TAAGATGTTAGAGTAGGGGGAGTCCAAGTCTCTGCTACCGTATAAGGTAGGAGGTTTGGTT5660.14642765671769215No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT5220.13504458799758887No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG4840.12521375592113604No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGCGTGAGGGTGTATATGTGGG4750.12288540095566038No Hit
ACGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG4620.1195222215610844No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTACATGTGGG4560.11796998491743396No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT4330.11201974445010723No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGCGTGAGGGTGTATATGTGGG4300.11124362612828201No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG4250.10995009559190663No Hit
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGCATATGTGGG4240.10969138948463157No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT4210.10891527116280635No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGCTGAAGTAG4120.10658691619733066No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTACATGTGGG3940.10193020626637933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGG151.6228214E-4135.237262
GTGTTAC100.008731476135.237268
ATCTTGT151.6228214E-4135.237265
TTCGTTG1550.0135.237267
GGTCTGA100.008731476135.237267
GGATCTT151.6228214E-4135.237263
TTCGAGC151.6228214E-4135.237264
GATAAGA3100.0135.237262
TATTGTA100.008731476135.237266
GTCTGAG100.008731476135.237268
ATGGATC100.008731476135.237261
CTTGGTT100.008731476135.237264
AGAGTTA203.0116262E-6135.237267
ACGAGTG100.008731476135.237265
AAGATGT1450.0135.237262
GATCTTG151.6228214E-4135.237264
CGTATTA100.008731476135.237269
GGCTTGA151.6228214E-4135.237267
TCTGAGG100.008731476135.237269
TACGAGT100.008731476135.237264
Produced by FastQC (version 0.11.5)