FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005577597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241122
Sequences flagged as poor quality0
Sequence length20-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG222279.218155124791599No Hit
GTGTTTGGTTTGAGGAGGGTGTGTGGGGTGTGTGAGGGTGTATATGTGGG204078.463350503064838No Hit
GTTTTTGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG171637.117973474009008No Hit
GTTTTCGAGTGGGTTTGGAGTTGTGGAGTTGGGTGGGGGTGGGAAGGAGG152556.326672804638315No Hit
GTTGTTATTTGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG128715.337961695739087No Hit
ATGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG102704.259254651172435No Hit
GTTGTTATTCGTAGGGGTATTGGTGGTGTTTGTTGTTAAGGGGTAGAGTG84323.4969849287912345No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG84203.492008195021607No Hit
ACGGATTTTGTGGGTGGAAGGAGATGTTGTAGTTGGAGTGGTTGAAGTAG83763.473760171199642No Hit
TTGTTAAGCGTTATTAATAGGTTGTATTGTTTTTTGTGGTTGTTGTGTGG71012.9449822081767736No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTAGTGTGAGAGTGTGGTG62052.573386086711291No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT49332.04585230713083No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTTGGGGAGTGGGGGTGGGGGGTT44911.8625426132829026No Hit
TCGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG42071.744759914068397No Hit
TTGGGGGTGGAGTGGGAGTGTGTGTGTGTGAGTGTGTGTGTGTTGTGGTG37571.5581323977073847No Hit
AATGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT28201.1695324358623436No Hit
TTTGTGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG27591.144234039200073No Hit
AACGTTGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT26961.1181061869095312No Hit
ATTTGTGTTAAGGGATAGGAGAAGCGGAGTTTGTTTTTTGGGTTTAGAAG24181.0028118545798392No Hit
TTTGCGTTTATAAGTTAAGTTGTTTATTTTGGTTGTGGTGGGAATTGTGG23830.9882963810850939No Hit
AATGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT21670.8987151732318079No Hit
AACGTCGTTTTAGAGAGATTAGGTTGGTTTTTGTTGTTGTTGTTGTTATT20920.8676105871716392No Hit
GTTTTTGAGTGGGTTTGGAGTCGTGGAGTTGGGTGGGGGTGGGAAGGAGG19710.8174285216612337No Hit
TGTGTTGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT18820.7805177462031669No Hit
GTTTTCGAGTGGGTTTGGAGTCGTGGAGTTGGGTGGGGGTGGGAAGGAGG18650.773467373362862No Hit
TAAGATGTTAGAGTAGGGGGAGTCCAAGTCTCTGCTACCGTATAAGGTAGGAGGTTTGGTT18570.7701495508497772No Hit
TAAGATGTTAGAGTAGGGGGAGTCCAAGTCTCTGCTACCGTATAAGGTAGGAGGTTCGGTT18170.7535604382843539No Hit
CGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT17930.7436069707451No Hit
TGTGTCGTATAAGGTAGGAGGTTTGGTTTTGGTATTGTTTAAGTTTTTTT16030.6648086860593393No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTAGTGTGAGAGTGTGGTGG16010.6639792304310681No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA11710.48564627035276753No Hit
ATTTAGTTCGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT10990.4557858677350055No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTTAATTTTGTA10530.4367083882847687No Hit
ATTTAGTTTGTTGGAGGTTTTGTATTTGGTGTGTAATTTTTTTTGAGTGT10280.42634019293137915No Hit
GGTATTTTAAAGTTGTTTGTATTTTGTTTTATTTGTAGGGGTAGGGGTTT8070.33468534600741534No Hit
TGTTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTT7580.3143636831147718No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTTATATTAGATAAT7530.3122900440440939No Hit
GGTTGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA7240.30026293743416194No Hit
ATGGATTTTGTGGGTGGAAGGAGACGTTGTAGTTGGAGTGGTTGAAGTAG7200.2986040261776196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6950.28823583082423004No Hit
GGTCGAGGGGGAGGAATTTGATTTTGGATGGTGAGTGGAGTTTTGTTGAA6710.2782823632849761No Hit
GGATAAGAGGTTTGTTGAAAGTTTATTTTTTTTTTTTATATTAGATAATG6670.27662345202843375No Hit
GCGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG6590.2733056295153491No Hit
GTGTTTGGTTTGAGGAGGGTGTGTNGGGTGTGTGAGGGTGTATATGTGGG5870.24344522689758713No Hit
ACGGATTTTGTGGGTGGAAGGAGACGTTGTAGTTGGAGTGGTTGAAGTAG5480.2272708421462994No Hit
GTTTTTGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG4780.1982398951568086No Hit
GTTTTCGAGTGGGTTTGGAGTTGTNGAGTTGGGTGGGGGTGGGAAGGAGG4380.18165078259138526No Hit
GTTGTTATTTGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG4040.16755003691077547No Hit
GGAGGGTGGGTTTTGTTTTGTAATTTTTTTTTTTAGTGTGAGAGTGTGGT3790.1571818415573859No Hit
TCGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT3720.15427874685843682No Hit
TTGGAGAGAGAATTTAATAGGTTTGGATGTATTTTTTTTTAATTTTGTAT3580.14847255746053864No Hit
ATGGATTTTGTGGGTGGAAGGAGANGTTGTAGTTGGAGTGGTTGAAGTAG3310.1372749064788779No Hit
GTGGGAGTTTATAAATTTTTATTAGTTTCGGGGAGTGGGGGTGGGGGGTT3270.13561599522233558No Hit
GCGGGAGTTTATAAATTTTTATTAGTTTCGGGGAGTGGGGGTGGGGGGTT3080.1277361667537595No Hit
GTTGTTATTCGTAGGGGTATTGGTNGTGTTTGTTGTTAAGGGGTAGAGTG2600.10782923167525153No Hit
TTGTTAAGTGTTATTAATAGGTTGNATTGTTTTTTGTGGTTGTTGTGTGG2480.10285249790562455No Hit
TTGTTAAGTGTTATTAATAGGTTGTATTGTTTTTTGTGGCTGTTGTGTGG2480.10285249790562455No Hit
CGTTTTTTTTTGAAGTTTTTTTAGAGGGTGTGTTAGGTTGTTTGGTTTTG2470.10243777009148897No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCA100.008857097134.582055
TGCGTTA100.008857097134.582053
TCGATGG100.008857097134.582055
TTCGATG100.008857097134.582054
TTATTCG9550.0134.582055
GTCTGGT100.008857097134.582053
CGAGGGG700.0134.582054
TCTTGAG100.008857097134.582053
GCGTTTC100.008857097134.582054
ATGTTCG100.008857097134.582055
TCGTATA1800.0134.582055
CTTGAGT100.008857097134.582054
GTTATTC9550.0134.582054
AGTTCGT1400.0134.582055
TTTCGAT100.008857097134.582053
GGATAAG2650.0134.582031
TGTCGTA1750.0134.582033
TTCGTAG9500.0134.554128
TCAGGGA100.00886259134.554129
TCGCGTA100.00886259134.554129
Produced by FastQC (version 0.11.5)