FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005577598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005577598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322391
Sequences flagged as poor quality0
Sequence length20-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAAATTTC3709711.506834868219027No Hit
AAAACCAACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAAATTTC3247010.071621106048244No Hit
ATACATCCAAACCTATTAAATTCTCTCTCCAACACACCCACTTCTAACAA240297.453371837303151No Hit
ATACGTCCAAACCTATTAAATTCTCTCTCCAACACACCCACTTCTAACAA231687.186304828608739No Hit
AAAAAACAACCCAACCTAAACAAACAAACAAAACCAAACAAAAAACAAAT119793.7156744450062194No Hit
AAAAAACAACCCGACCTAAACAAACAAACAAAACCAAACAAAAAACAAAT108683.371061847260004No Hit
CCGCCCCTTTCTCCATAAATAAAAAAAAAACCCCTATTTAAAAAAATCTC80182.4870421320694436No Hit
CCACCCCTTTCTCCATAAATAAAAAAAAAACCCCTATTTAAAAAAATCTC75372.337844418733774No Hit
GTCTCCTTCCACCCACAAAATCCATCCCCAACAAACAAACAATAACTCAA52941.6421053937609922No Hit
ATCTCCTTCCACCCACAAAATCCATCCCCAACAAACAAACAATAACTCAA50101.5540136045981432No Hit
TCCGAAATCAAATTCCTCCCCCTCAACCCAACCAAAAAACACCAACAAAA38411.1914104301919097No Hit
CCGCCCCTTTCTCCATAAATAAAAAAAAACCCCTATTTAAAAAAATCTCC37821.1731096711756843No Hit
ATAAAACAAAATACAAACAACTTTAAAATACCAACATTCAACTCCCTACA37661.1681467534763688No Hit
AAAAACCACTTAAAAACCTCAAACACAAATTAACAACAAACACTAAAAAA37291.1566700062967017No Hit
TCCAAAATCAAATTCCTCCCCCTCAACCCAACCAAAAAACACCAACAAAA36641.136508153143233No Hit
CCACCCCTTTCTCCATAAATAAAAAAAAACCCCTATTTAAAAAAATCTCC36561.1340266942935753No Hit
CCCGAAACTAATAAAAATTTATAAACTCCCACAACAACCCCCAAATTATC35721.1079713763721692No Hit
CTATTAATAACGCTTAACAACTACAAAAACACCCACAATCCCTACCCCCA35581.1036288233852682No Hit
TAACACGCCCTCTAAAAAAACTTCAAAAAAAAAACAAAATAACAAAAATC35311.0952538997676735No Hit
CTATTAATAACACTTAACAACTACAAAAACACCCACAATCCCTACCCCCA33651.0437636286372758No Hit
AACTTTCAACAAACCTCTTATCCAAAAACAATCTACAAAAACCACCTCCA30710.9525700159123549No Hit
CCCAAAACTAATAAAAATTTATAAACTCCCACAACAACCCCCAAATTATC30400.9429543628699312No Hit
TAACACACCCTCTAAAAAAACTTCAAAAAAAAAACAAAATAACAAAAATC23450.7273776253059173No Hit
TAACACGCCCTCTAAAAAAACTTCAAAAAAAAACAAAATAACAAAAATCA23130.7174517899072865No Hit
AAACTCGCTCTACCCCTTAACAACAAACACCACCAATACCCCTACAAATA22860.7090768662896917No Hit
CCCCCTACTCTAACATCTTAAAAACAATTAAAAAAACACATCCCAAAAAC21380.663169877571024No Hit
AAACTCACTCTACCCCTTAACAACAAACACCACCAATACCCCTACAAATA21010.651693130391357No Hit
TAACACACCCTCTAAAAAAACTTCAAAAAAAAACAAAATAACAAAAATCA15610.4841946580394614No Hit
TACCGACAAATCCCCCACCACCACATCATCTAAACAACCAAATCTTCAAC13230.4103712572621444No Hit
TACCAACAAATCCCCCACCACCACATCATCTAAACAACCAAATCTTCAAC12020.3728391921610715No Hit
CTATTAATAACGCTTAACAACTACAAAAACACCCACAATCCCTACCCCCC11190.3470940565958727No Hit
NAAACCGACCTANTCTCTCTAAAACAACATTANAAAAACCNCCAAATNTC10250.3179369151123946No Hit
CTATTAATAACACTTAACAACTACAAAAACACCCACAATCCCTACCCCCC9610.29808524431513284No Hit
ACGCCCTCCTCAAACCAAACACCACCAAAACCTCCCCAAAACCCAAACTC9410.2918815971909886No Hit
NAAACCAACCTANTCTCTCTAAAACAACATTANAAAAACCNCCAAATNTC8730.270789196968898No Hit
NTACGTCCAAACNTATTAAATTCTCTCTCCAANACACCCANTTCTAANAA6590.20441017274055417No Hit
NTACATCCAAACNTATTAAATTCTCTCTCCAANACACCCANTTCTAANAA6510.20192871389089645No Hit
CCGCCCCTTTCTCCATAAATAAAAAAAAAAACCCCTATTTAAAAAAATCT6000.18610941372432854No Hit
NAAAAACAACCCNACCTAAACAAACAAACAAANCCAAACANAAAACANAT5900.1830075901622564No Hit
ACACCCTCCTCAAACCAAACACCACCAAAACCTCCCCAAAACCCAAACTC5780.1792854018877698No Hit
ACGACAAACTTAAAAACAAATTACAAACAAAATAATAAATTACTAACAAA5460.16935956648913897No Hit
CCACCCCTTTCTCCATAAATAAAAAAAAAAACCCCTATTTAAAAAAATCT5370.16656792528327405No Hit
AAAACCGACCTAATCTCTCTAAAACGACATTAAAAAAACCACCAAATTTC5270.16346610172120188No Hit
AAAACCAACCTAATCTCTCTAAAACGACATTAAAAAAACCACCAAATTTC5250.16284573700878746No Hit
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAACTTTC4990.1547809957473999No Hit
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAGACCACCAAATTTC4860.15074862511670611No Hit
AAAACCAACCTAATCTCTCTAAAACAACATTAAAAAGACCACCAAATTTC4310.13368859552530934No Hit
ACAACAAACTTAAAAACAAATTACAAACAAAATAATAAATTACTAACAAA4260.13213768374427326No Hit
AAAACCAACCTAATCTCTCTAAAACAACATTAAAAAAACCACCAACTTTC4240.13151731903185884No Hit
ATACATCCACACCTATTAAATTCTCTCTCCAACACACCCACTTCTAACAA3380.10484163639803842No Hit
AAAACCGACCTAATCTCTCTAAAACAACATTAAAAAACCACCAAATTTCT3360.10422127168562398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)