FastQCFastQC Report
Fri 10 May 2024
EGAF00005578192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11393179
Total Bases837 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG4287543.7632516789212214No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2519342.211270445237453No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG926540.8132409751483761No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC723310.6348623154257472No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG600880.5274032822621325No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG520250.4566328677887006No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG510720.448268213814599No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT411880.36151455182087455No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC303140.2660714801373699No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG303010.2659573767778071No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279650.24545388078252786No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT261440.22947063326223524No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG258360.22676726135874808No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA227150.19937367788217847No Hit
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT207890.18246882630387884No Hit
GTTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGC206460.18121368934868837No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG202000.17729906639753487No Hit
GGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACT201040.17645645697307136No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA188600.16553764318106473No Hit
TGGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTAC181410.1592268496790931No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA179690.1577171744602626No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTG175400.15395176359469118No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA174660.15330225216333387No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG173650.1524157568313462No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG171900.1508797500680012No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG167500.14701779020587669No Hit
TTTGGCTATAATTTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG166760.14636827877451938No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA164400.14429686393937988No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG163750.143726347141566No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC162370.1425150960938997No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG161780.14199724238511482No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA159520.14001359936502358No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG155530.13651150394459702No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG152630.13396612130819677No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC149600.13130663531223374No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG146260.12837505668962104No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA141770.12443410219395307No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA137470.12065991414687684No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA137200.12042293024624647No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT136170.11951888055124914No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG135260.11872015703430973No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA134770.11829007514057314No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA134540.11808819996596209No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC130320.11438422937092448No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG127810.11218115681321254No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG127100.11155797692636972No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT126140.11071536750190618No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG123270.10819631641002042No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG123180.10811732177647697No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA122560.1075731365231776No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG119730.10508919415731113No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG117190.10285979005508472No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG116160.10195574036008738No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA115000.1009375873055273No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC114980.10092003294251761No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG114710.10068304904188727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)