FastQCFastQC Report
Wed 8 May 2024
EGAF00005578204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10574141
Total Bases797.2 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT836350.790938951920539No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT688950.6515422860353385No Hit
GGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACT509990.4822992241166445No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT432930.4094233280982351No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT395180.3737230286602004No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA362070.34241079251733075No Hit
TGGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTAC358870.339384541968941No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC345180.32643786384161133No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG337910.3195626008769885No Hit
GTTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGC337260.31894789373434684No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA329880.3119686034071231No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA328590.3107486461548035No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA314410.29733857341225167No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA288290.2726368033110207No Hit
TTTGGCTATAATTTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG274960.2600305783703849No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA259730.24562751716664266No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC257930.24392525123317346No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT251350.2377025235430471No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA242240.22908716651310024No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATT228680.21626342981429886No Hit
TGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTA227040.21471247640824914No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG208130.19682922707385878No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG198860.18806255751649237No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA195090.18449725608917075No Hit
TTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTA193540.18303141597979447No Hit
TGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA186210.17609941081738933No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG178330.1686472688419797No Hit
TTGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGC171020.16173417774550197No Hit
GGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA169480.16027779466908945No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC168800.1596347164275566No Hit
CGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCT163180.1543198639019472No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA162800.15396049664932593No Hit
GTTGGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGC155970.14750134313510666No Hit
GGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTAT155330.14689609302542872No Hit
GGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTCC146690.13872521654477654No Hit
TGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTAG146230.13829019302844553No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142810.13505588775485403No Hit
GTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACTT134010.12673369874678236No Hit
GAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATAC131240.12411410061583253No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG130910.12380201852802983No Hit
CTCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGC130460.12337645204466255No Hit
GGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT130330.12325351061613422No Hit
TTCGTTATTGAGTTTAGGTCCTAACAATGAATTTTCACATATAAGTTGGA127490.12056771325443835No Hit
AGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT127090.12018943193588963No Hit
GGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATTCAC126760.11987734984808694No Hit
TCATTGCGCCACGGCGGCTTTCGGACGAGCCCCTGACTCGCGCACGCGTT125800.11896947468357003No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA119980.11346548149868628No Hit
TGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG119420.11293588765271807No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT119270.11279403215826232No Hit
GGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAG111160.10512437842468718No Hit
GCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATTTGGG108160.10228726853557184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)