FastQCFastQC Report
Wed 8 May 2024
EGAF00005578213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19376850
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230440611.892572838206416No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG889990.4593058211215961No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG417880.2156594080049131No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC371200.19156880504313137No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT324300.16736466453525728No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT265060.136792099851111No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG241920.1248500143212132No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT241280.12451972327803537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG210610.10869155719324866No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG209310.10802065351179371No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT203890.1052235012398816No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT195070.10067167780108738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)