FastQCFastQC Report
Wed 8 May 2024
EGAF00005578217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16677928
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15346149.20146675294437No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG661550.39666198343103526No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT658020.39454541355496914No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG373980.22423648788986258No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT325000.1948683313658627No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT316170.18957390870136867No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG302080.18112561704307634No Hit
GGAACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT252840.15160156585398377No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT245980.14748834507499972No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG206920.12406816961915174No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT184660.11072118790775448No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG180830.10842473957196602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)