FastQCFastQC Report
Wed 8 May 2024
EGAF00005578228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16652684
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN230129813.819381908646077No Hit
TGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCC794580.47714830834476896No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG614820.36920174549640167No Hit
AGCTGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCC526270.31602713412444505No Hit
GAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCG491470.2951296019308359No Hit
GAGGTGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCG484660.2910401710619141No Hit
TGCTGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCC274250.16468816678440543No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253710.1523538187597867No Hit
AGCCTCGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCC245440.1473876523448112No Hit
TGCCTCGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCC230700.13853622635246066No Hit
GCTGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCG221930.1332698080381517No Hit
CGCCCTGGGAAAACCACCTTCGTGATCATGGTATCTCCCCTGCCCCC218970.13149231679409756No Hit
GGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCC200500.12040101163271939No Hit
AGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCC187140.11237828088252921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)