FastQCFastQC Report
Wed 8 May 2024
EGAF00005578234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15546932
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7419444.772285618796043No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1017510.6544763944423247No Hit
TGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCC242330.1558699812927721No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT223610.14382902041380252No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG218340.14043928409798154No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG190280.12239070705397051No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG188860.12147734356849312No Hit
AGCTGAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCC159100.10233530319679793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)