FastQCFastQC Report
Fri 10 May 2024
EGAF00005578237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11098144
Total Bases748.9 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10100989.101503819016946No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC1568381.413191250717237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA994790.8963570845719788No Hit
ATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA898560.8096488926436709No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC852140.7678220790791686No Hit
TTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA755700.6809246663225851No Hit
TTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680630.6132827254719347No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT677280.61026420273516No Hit
ATTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA535990.4829546273683239No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA505220.4552292707681572No Hit
ATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA499690.45024645562357096No Hit
TTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493690.4448401462442729No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT464800.41880876658295296No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC438620.3952192366579493No Hit
CGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT418040.3766755954869571No Hit
ATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA394050.3550593684853972No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT388430.3499954587001214No Hit
TTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA351440.3166655613767491No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT347960.3135299019367563No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT306320.27601011484442806No Hit
GGGGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGA298880.2693062912140985No Hit
GGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA295610.2663598526023811No Hit
AGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA292880.26389998183480046No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC265030.23880569579922553No Hit
CCGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC254010.22887610757258153No Hit
ATTAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250250.2254881536948881No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG248520.22392933449052382No Hit
GGGGGGCGGAGGAAGCTCATCAGCGGGGCCACGTGCTGAGTGCTCGTCAC235930.21258509530963013No Hit
GCGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC232770.20973777236986654No Hit
GGGAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230330.2075392065556187No Hit
GCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCG227920.2053676722882673No Hit
TTTAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA221860.19990729981517633No Hit
AGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA213470.19234747719979126No Hit
AGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA196510.1770656426876422No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT195830.1764529276246551No Hit
AATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195370.17603844390557555No Hit
GGGGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG192570.17351549952856982No Hit
GGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC192160.17314606838765112No Hit
CGGGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGA190260.17143407041754008No Hit
GGGGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCAC189250.17052400833869158No Hit
GCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT188300.16966800935363607No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT184560.16629807650720696No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT184290.16605479258513856No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180630.16275694386376677No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG178600.16092780919043762No Hit
CGGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT176090.15866616976676462No Hit
ATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175920.15851299100101784No Hit
GTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175460.1580985072819383No Hit
GGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175450.15808949676630615No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT174920.1576119394378015No Hit
GGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA174230.15699021385918221No Hit
CCGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT173160.15602608868654075No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC167270.15071889497919652No Hit
CGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC165870.14945742279069363No Hit
GCGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT161900.1458802480847248No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA158580.14288875689484656No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155780.1403658125178408No Hit
CGGGGGCGGAGGAAGCTCATCAGCGGGGCCACGTGCTGAGTGCTCGTCAC154370.1390953298137058No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC148430.13374308352820075No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG145160.13079664491648335No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC142710.1285890685866033No Hit
CGGCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGAT141340.1273546279449969No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT140350.12646258689741277No Hit
TTAAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA133430.12022731007995571No Hit
AGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131750.11871354345375226No Hit
TATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130020.117154724249388No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129760.11692045084295176No Hit
AATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129710.11687539826479094No Hit
GGGGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG128940.11618158856111438No Hit
AGGAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126240.11374874934043026No Hit
GGGGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGGGTGCG124510.112189930136066No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT121000.10902723914917666No Hit
ATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116940.10536896980251832No Hit
AATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116780.10522480155240371No Hit
CCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT116530.10499953866159964No Hit
CGGGGAACGGGACGGGAGCGGCCGCGGGTGCGCGTCTCTCGGGGTCGGGG116160.10466614958320958No Hit
TGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114410.103089309347581No Hit
AGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112870.10170168994022785No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111850.10078261734574717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)