FastQCFastQC Report
Wed 8 May 2024
EGAF00005578241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913426
Total Bases367.9 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG3092396.293755110995872No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2119104.31287659567886No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1342472.732248333443915No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1325022.6967333994650575No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1086912.212122457934647No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG853581.7372399625027426No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC848961.727837154767366No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT451540.9189921655480311No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT409010.8324334181485586No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC399030.8121217252483298No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC343060.698209355345944No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT319940.6511546118736702No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC278800.567424847753889No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC274170.5580016876208169No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN245110.49885761991734484No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC237040.4824332349769794No Hit
GCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT229490.46706717471678616No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC217900.44347874578756247No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC205650.4185470586104278No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC192450.39168189365220923No Hit
CGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC185410.3773538056744927No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT170160.346316399188672No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT160780.32722585015018035No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC155090.3156453358613725No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT154930.31531969749824257No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG149600.30447186952647703No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT138520.28192141287972994No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC133280.271256756487225No Hit
GGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC130450.26549702793936447No Hit
GGGGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC129750.26407236010067114No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC128210.2609380908555456No Hit
CCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT126860.2581905171666369No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT126580.2576206500311595No Hit
GGCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT123230.2508025968031268No Hit
CGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG109110.2220650112569112No Hit
CGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG108030.21986695230578418No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA98590.2006542888811188No Hit
GGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG98480.20043041250646698No Hit
GCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCG98110.19967737379172906No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT91060.18532893341631684No Hit
GGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG86520.1760889448625053No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG82770.16845679572664776No Hit
CGGGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC78820.16041759863687782No Hit
GGGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG77260.15724262459636107No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT77070.15685592904014428No Hit
CGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG71400.14531611954672768No Hit
GGCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG68010.13841665672791245No Hit
CGCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT66370.13507886350583076No Hit
CCCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCG62750.12771129554001628No Hit
CGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC61880.1259406369404973No Hit
CGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC61380.12492301705571633No Hit
GGGGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGC61130.1244142071133258No Hit
GGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC60510.12315235845619737No Hit
GCGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCG59510.12111711868663536No Hit
CCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC59120.12032337517650617No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT58120.11828813540694415No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC57860.11775897306685802No Hit
CCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT56850.11570338089960042No Hit
GGGGGGAGGCTGAGACAGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG56310.11460435142403691No Hit
GGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC54780.11149043457660704No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC54010.10992329995404429No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53650.10919061363700196No Hit
CCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT51030.1038582854407495No Hit
GGGGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGA50780.10334947549835898No Hit
CGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG50080.10192480765966558No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT49680.10111071175184078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)