FastQCFastQC Report
Wed 8 May 2024
EGAF00005578271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10259176
Total Bases767.9 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1080651.053349703718895No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1060961.034157129188543No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC869110.847153806504538No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN733480.7149502065273078No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC695260.6776957525633638No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG582210.5675017174868625No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA529200.5158309010392258No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG391940.3820384795036171No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC390860.3809857633790472No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC380940.3713163708274427No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT377080.3675538854192579No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC300290.29270381948803686No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG288380.28109470000319714No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC267220.2604692618588471No Hit
GGGCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC255640.24918180563429265No Hit
CGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC244160.2379918231249761No Hit
GGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT241400.23530154858440872No Hit
GCCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC240970.23488241160888554No Hit
CGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC220720.2151439842732009No Hit
GGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC217430.21193709904187238No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC216420.21095261451796907No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC213830.20842804529330622No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT207960.2027063382088386No Hit
CGGCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC207660.20241391706312478No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT200580.19551277802427797No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC177720.17323028672088284No Hit
GGGTGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC174160.16976022245841185No Hit
CGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA173170.16879523267755617No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT170950.1666313161992737No Hit
GGCCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC170110.16581253699127493No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG169440.16515946309918067No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA160940.15687419730395502No Hit
CGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG156570.15261459594805665No Hit
CCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT152270.14842322619282483No Hit
GGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT149710.14592789908273335No Hit
GGGCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG144170.14052785525855097No Hit
CGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC144030.14039139205721785No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT137850.13436751645551262No Hit
GGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC137690.1342115585111319No Hit
CCGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC135880.132447284265325No Hit
CGGTGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC133690.13031260990161395No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT132990.1296302938949483No Hit
GGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC132770.1294158517214248No Hit
CGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC131290.12797324073590316No Hit
CGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT126350.1231580392031485No Hit
GCGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC125560.12238799685276869No Hit
GGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGCG125130.1219688598772455No Hit
GGCTGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC118350.11536014198411255No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC116940.11398576259925748No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC116710.11376157305421021No Hit
GGCGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT105860.10318567495089274No Hit
GGCCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA105730.10305895912108341No Hit
CGCACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT103410.100797568927563No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)