FastQCFastQC Report
Wed 8 May 2024
EGAF00005578273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11667256
Total Bases829.6 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4861534.166815230590638No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC781290.6696433163033364No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC739340.6336879897038344No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT702040.6017181760647061No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG691720.5928729085913603No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC634100.543486831865179No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC561030.48085856691581985No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT434080.3720497775998058No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC429520.3681414035999553No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG404800.3469539024428709No Hit
CGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTC311460.26695222938452706No Hit
CCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT284740.24405052910470124No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC272340.23342249454370417No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT261360.2240115413598536No Hit
CGGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC251270.21536340678562296No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC244040.20916657695691257No Hit
GGGGAAGGGCCCCGCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCA218370.187164831216526No Hit
CGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC211410.1811994182693857No Hit
CGGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCC200680.1720027399758778No Hit
GGCGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT199370.17087993955048214No Hit
GGGCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC198570.17019425990138556No Hit
CGGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGC196520.16843720580057556No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC189390.16232608592800227No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG189230.16218894999818295No Hit
GGGGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCC189120.16209466904643216No Hit
GGGGGGCCCTTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGCCGC186590.1599262071561642No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG183600.15736347946766577No Hit
CGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG183150.15697778466504891No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG173900.1490496137223697No Hit
CCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG173170.14842393104256904No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC169380.145175523704974No Hit
CGGGGGCCCTTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCGGCCGC168360.14430128215237586No Hit
CGGCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC165430.1417899804375596No Hit
CGGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGT160190.137298778735977No Hit
GGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC159320.13655310211758445No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC152650.1308362480432417No Hit
GGGGGAACGATACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGC152620.13081053505640058No Hit
GGCCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC151480.12983344155643795No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC151090.12949917272750336No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG149310.12797353550826346No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC139460.1195311048287618No Hit
GGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG138920.1190682710656216No Hit
GGGGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTC135790.11638554943853123No Hit
GGGAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGT135230.11590557368416361No Hit
GCCGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT134140.11497133516226953No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA133220.11418280356580844No Hit
GGGGAGAGATGGGCGAGTGCCGTTCCGAAGGGACGGGCGATGGCCTCCGT132980.11397709967107947No Hit
GGGGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGG132540.11359997586407634No Hit
GGGGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGC128830.11042013649139094No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG127320.10912591615372116No Hit
GGCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG124660.10684603132047502No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA123480.10583465383805755No Hit
CGGGAAGGGCCCCGCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCA122480.10497755427668683No Hit
GGCACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC120700.10345191705744693No Hit
CGGGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCC118730.10176343092154659No Hit
GGCAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGT118440.10151487204874908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)