FastQCFastQC Report
Fri 10 May 2024
EGAF00005578286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10932506
Total Bases818.7 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2539792.323154453333938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN775510.7093616047409441No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA739590.6765054599558418No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC519870.4755268371222481No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG517490.47334984311922623No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT495590.4533178394779751No Hit
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT467250.4273951461814885No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG371980.34025135682523294No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG367940.33655595524027154No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC352650.3225701408259003No Hit
GGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACT317240.2901804947557312No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG283940.2597208727806781No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG283730.2595287850745291No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT279620.25576935425418473No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA273750.2504000455156393No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT256040.2342006489637417No Hit
GTTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGC251470.2300204545965948No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT240630.22010507014585678No Hit
TGGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTAC239890.21942818965752225No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC231040.2113330648983865No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA226660.2073266641701363No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA221330.20245129524740255No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA214320.196039224675477No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC204630.18717574909174528No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA200520.1834163182714009No Hit
TCATTGCGCCACGGCGGCTTTCGGACGAGCCCCTGACTCGCGCACGCGTT197090.18027888573763418No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA195360.1786964489203116No Hit
TTTGGCTATAATTTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG195120.17847692011328417No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT190100.17388510923296085No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA182540.16696995181159746No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG182230.16668639376918706No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT181880.16636624759227206No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG180160.16479295780857564No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC176070.16105182105548352No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA157270.14385539783833642No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA156870.14348951649329075No Hit
TGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTA155940.14263884236605953No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA155920.14262054829880724No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG150030.13723294549300957No Hit
TTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTA135610.1240429230041127No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC133830.1224147510186594No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATT131560.12033837438552514No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC129570.11851811469392289No Hit
GGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTAT123060.11256339580330438No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA122990.1124993665679214No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG122300.11186822124771759No Hit
TGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA121660.11128281109564449No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG116270.10635255997115392No Hit
GGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA112730.10311451006749961No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC112260.10268459948707094No Hit
TGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTAG111820.1022821300075207No Hit
TTGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGC110950.10148633808204632No Hit
CGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCT109640.10028807667702172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)