FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756368
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT161970.4311877856482645No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT91020.24230852781197157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC79750.21230614252916646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67990.18099930571232636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66980.17831053826462157No Hit
CTACAAACGTTCGCTTTCGGCCCTGGGACCAAACTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT64200.1709097724184638No Hit
GATTTTACACTGAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA63980.17032410030114195No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG59830.15927619445166183No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA58480.155682297368096No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG56660.1508371916702517No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG54100.14402209794141574No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAATCTGTCAGGAACCCCG54100.14402209794141574No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT52410.1395230712219889No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50450.1343052650858489No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT50370.13409229340682274No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG47900.1275167928168912No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45680.12160682872391629No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG45030.11987643383182904No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44830.11934400463426374No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43570.11598970068960231No Hit
GGTCTAGTCAGAGTCTCCTGCATAGAAATGGATTCAGTTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC42260.11250228944554953No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAGTTTGGTCC40790.10858893484344452No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40720.10840258462429665No Hit
CGTTTGTAGAGCTTGCATGCAATAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAAAAT40560.10797664126624441No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAGTTTGGTCCCAGGGCCGAAAGCG40250.10715137601001819No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG40220.10707151163038338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG32450.048.69046869
TCGTATG32400.038.64332640
CGTATGC32850.038.29859541
TATACAC37850.037.6873
GCCGTCT32850.037.41431446
CTTATAC37300.037.275151
ATGCCGT33450.036.89052244
TGCCGTC33450.036.70767245
TAAGGCG37550.036.5672629
AATCTCG32900.035.88930536
GCGAATC34900.035.87986833
ACGAGAC38350.035.8675222
CTCGTAT33200.035.78079239
GGTATCA55100.035.6975631
CCGTCTT35000.035.63860747
GTATCAA128950.035.310391
GACTAAG39250.035.09153726
ATCTCCG39450.035.0710410
GGCGAAT38450.034.8989332
CGAGACT39500.034.758323
Produced by FastQC (version 0.11.5)