FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399057
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235891.6860642561382417No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41000.29305453601961895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30570.21850432112487198No Hit
GATCTATGCTGCATCCACTTTGGAAAGTGGGGTCCCATCAAGGTTCAGGGGCAGTGGATCTGGGACAGATTTCA27980.1999918516543643No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26320.18812671678137488No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25920.18526764813728105No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25190.18004984786180978No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23680.1692568637303555No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23250.16618336493795463No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22700.16225214555232562No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22460.16053670436586928No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21510.1537464163361464No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCGGACCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA21400.15296017245902063No Hit
GTTCAGGGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCATCAGTCTGCAGCCTGACGATTTTGCAACTTA21040.15038701067933616No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCGGACCATTAGCAG20630.14745646531913997No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19750.1411665143021335No Hit
GGATACTGTGACTGTGTTGACAGTAGTAAGTTGCAAAATCGTCAGGCTGCAGACTGATGATGGTGAGAGTGAAAT19670.14059470057331472No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18970.13559133044615052No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18660.13337555224697778No Hit
GCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATT18560.13266078508595433No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGATA18430.13173158777662383No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18410.13158863434441914No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18200.13008762330626986No Hit
GTAGTAAGTTGCAAAATCGTCAGGCTGCAGACTGATGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCC18040.12894399584863234No Hit
GATTTCACTCTCACCATCATCAGTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACACAGTCACAGTATC17750.1268711710816643No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17630.12601345048843612No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17610.12587049705623146No Hit
ACTTACTACTGTCAACACAGTCACAGTATCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA17450.12472686959859391No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17330.12386914900536575No Hit
CAGTATCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT16930.12101008036127191No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16470.11772215142056398No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15980.11421979233154904No Hit
GTCTGCAGCCTGACGATTTTGCAACTTACTACTGTCAACACAGTCACAGTATCCCGCTCACTTTCGGCGGAGGGA15790.11286173472560446No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15700.11221844428068335No Hit
CCCCTAAGCTCCTGATCTATGCTGCATCCACTTTGGAAAGTGGGGTCCCA15480.11064595652643174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15180.10850165504336134No Hit
GATCAGGAGCTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAAT14910.106571783708598No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14880.10635735356029097No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14710.10514224938655108No Hit
CTCCTGATCTATGCTGCATCCACTTTGGAAAGTGGGGTCCCATCAAGGTT14500.10364123834840182No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCCCTGAACCTTGATGGGACCCCA14440.10321237805178773No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14310.10228318074245724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)