FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968380
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140860.7156138550483139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65050.037.1268581
TATCAAC70450.033.859612
GGTATCA28200.033.5380971
TCAACGC72450.032.2614484
ATCAACG73500.032.1242373
AACGCAG73950.031.7454996
CAACGCA73650.031.7374135
ACGCAGA79550.029.5099937
AGAGTAC81600.028.97603211
CAGAGTA84600.027.94638410
CGCAGAG85300.027.638678
GCAGAGT85950.027.349789
AGTACGG65450.026.95343813
GAGTACG68000.026.09149612
TACGGGG34750.025.63194515
GTGGTAT11550.024.8580111
TGGTATC11600.024.7231862
GTACGGG73000.023.88855214
ACGGGGT10100.022.7205816
CGCAGTA1052.696724E-522.50812518
Produced by FastQC (version 0.11.5)