FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614708
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT131430.5026565107843782No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87040.3328861195972935No Hit
GTCATAGGATTAGTTCCTATTTGAATTGGTATCAACAAAAACCAGGGAAAGCCCCTGAACTCCTGATCTATGCTG67770.25918764160280994No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC66680.2550189160701692No Hit
GAGTTACGGTGCCCCTCCGACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCAT65740.2514238683631212No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG57190.2187242323043338No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56560.21631478543684418No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51770.19799534020624865No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT47360.18112921213382144No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47220.18059377949660155No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATAGGATTAGTTCCTATTTGAATTGGTATCAACAAAAA46550.17803135187562052No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG46310.17711346735467212No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46220.17676926065931645No Hit
GATCTATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTAGGTCAGATTTCA44500.1701910882591861No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG42230.161509430498549No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42150.16120346899156618No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41640.15925296438455078No Hit
CCGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGAC41570.15898524806594083No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41110.15722596940078967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40590.15523721960540143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39620.1515274363332349No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38610.147664672307577No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCCCTTGGCCGAAGGTC38570.14751169155408558No Hit
ACTTACTACTGTCAACAGAGTTACGGTGCCCCTCCGACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAA38290.14644082627964575No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGACCTAGATCCACTGCCA37500.14341945639819054No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCC36880.14104825471907378No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATAGGATTAGTTC35960.13752969738877152No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTT35900.1373002262585344No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT35720.1366118128678231No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34410.13160169319097964No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG33820.12934522707698143No Hit
GGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC33150.12678279945600043No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32840.12559719861644206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31700.12123724714193708No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC31140.11909551659305742No Hit
GTTCAGTGGCAGTGGATCTAGGTCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA31070.11882780027444746No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTCAAAT30900.11817763207210902No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT30110.11515626219065378No Hit
TCCTATGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA29460.11267032494641849No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT29460.11267032494641849No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28610.10941948393472618No Hit
CTCGTGTCCCTTGGCCGAAGGTCGGAGGGGCACCGTAACTCTGTTGACAG28490.10896054167425197No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGTTGATACCAATT28430.10873107054401486No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28290.10819563790679494No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATA26800.10249710483924018No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26740.10226763370900308No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC26250.10039361947873338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG27150.044.53854869
CTTATAC28450.039.8928531
TATACAC29750.038.370973
TCGTATG26350.038.10621640
CGTATGC26800.037.69071641
TTATACA30800.037.3906142
CGAGACG30850.036.40341623
GCCGTCT26750.036.25991446
ACGAGAC31150.036.14484422
TGCCGTC28150.036.12627845
AATCTCG27550.036.10252436
ATCTCGT27150.036.0796237
CGCTCAT31500.035.81755428
ATGCCGT28400.035.4607344
CCCACGA31700.035.39679319
ATCTCCG31750.035.21300510
CCGTCTT27650.035.16060347
GAGACGC32300.034.88624
CACGAGA32300.034.6445421
CGCGTAT709.65978E-734.53759439
Produced by FastQC (version 0.11.5)