FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2614708
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301691.1538190880205361No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT87880.3360987154206129No Hit
GTCATAGGATTAGTTCCTATTTGAATTGGTATCAACAAAAACCAGGGAAAGCCCCTGAACTCCTGATCTATGCTG65230.24947336375610585No Hit
GAGTTACGGTGCCCCTCCGACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCAT63610.24327764323970402No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60140.2300065628743248No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53790.20572086825756453No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52540.20094021971095816No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51980.19879848916207854No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48310.18476250502924227No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATAGGATTAGTTCCTATTTGAATTGGTATCAACAAAAA44790.17130019872199878No Hit
GATCTATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTAGGTCAGATTTCA43240.1653721945242069No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43060.1646837811334956No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42820.16376589661254717No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42240.16154767568692183No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG41580.15902349325431367No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG41220.15764666647289105No Hit
CCGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGAC39380.1506095518122865No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39330.15041832587042225No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38210.14613486477266296No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37970.14521698025171453No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37820.14464330242612175No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTT37570.1436871727168005No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37450.1432282304563263No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGACCTAGATCCACTGCCA36790.14070404802371814No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCCCTTGGCCGAAGGTC36170.13833284634460138No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCC35570.13603813504223033No Hit
ACTTACTACTGTCAACAGAGTTACGGTGCCCCTCCGACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAA35470.13565568315850185No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATAGGATTAGTTC34920.13355219779799504No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34780.13301676516077512No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA33760.12911575594674432No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33330.12747121284671176No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT32410.12395265551640947No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG31420.12016638186749726No Hit
GGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC31250.11951621366515879No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT30550.11683905047905922No Hit
GTTCAGTGGCAGTGGATCTAGGTCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA30460.11649484378370357No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTCAAAT29880.11427662285807823No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC29660.1134352287138755No Hit
TCCTATGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA28820.11022263289055603No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT27720.10601566216954246No Hit
CTCGTGTCCCTTGGCCGAAGGTCGGAGGGGCACCGTAACTCTGTTGACAG26330.10069958098571619No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26160.10004941278337771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)