FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033021
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT123010.40556923278803547No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA87130.2872713377190596No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79890.2634007479671258No Hit
ATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT73160.24121165003473435No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62140.20487823856148707No Hit
GTATAATATTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTC55970.18453548458780866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53390.1760291142065947No Hit
GTATAAAACACTCTGACTGGACTTGCAGTGGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA50590.16679739441302913No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49720.16392896719145697No Hit
CACTATCACCAGCCTGCAGGCTGAAGATGTGGCAGTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTT48010.15829102403181516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46230.1524222878773342No Hit
GTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAA45600.15034515092378192No Hit
GTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACTATCACCAGCC43830.14450938519713513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43210.14246521867141704No Hit
ACTTAGGAGGCTGGCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT42600.14045402257353312No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42400.13979461401684987No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40540.1336621144396956No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT40290.13283785374384155No Hit
CTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGA40250.13270597203250487No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39840.13135418449130423No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT39490.13020021951710853No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37970.12518871448631577No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37940.1250898032028133No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37690.12426554250695925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37680.12423257207912508No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36820.12139711528538707No Hit
GTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA36720.12106741100704546No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGAC33200.10946182040942017No Hit
CAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA32530.10725280174453129No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32390.10679121575485301No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG31850.10501081265180821No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31310.10323040954876342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91650.039.6790731
AAGGGGG25000.039.0903469
CTTATAC24750.036.7672461
TATCAAC101150.036.0815352
TCGTATG25350.034.80240
ACGCGTA25050.034.57099527
CTCGTAT24700.034.37226539
TCAACGC106450.034.312784
TATACAC26750.034.264073
ATCAACG106750.034.249613
ATCTCGT24550.034.1345537
CGTATGC26100.034.01683841
CGCGTAG25550.033.93096528
GACGCGT25600.033.9255926
CAACGCA108600.033.695535
AGACGCG26000.033.5037225
GCGTAGT26050.033.4429429
TATGCCG26950.033.21352843
AACGCAG110800.033.0867656
AATCTCG25300.033.05766336
Produced by FastQC (version 0.11.5)