FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033021
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254490.8390644179516066No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78520.25888379935384553No Hit
ATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT78200.2578287456631524No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA77420.2552570522920876No Hit
GTATAATATTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTC68320.22525396296299963No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53460.1762599072014338No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48360.15944498900601084No Hit
GTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAA47650.15710408862978528No Hit
GTATAAAACACTCTGACTGGACTTGCAGTGGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA47430.15637873921743373No Hit
GTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA46850.15446645440305226No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44870.14793830969188806No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44340.14619087701667743No Hit
CACTATCACCAGCCTGCAGGCTGAAGATGTGGCAGTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTT43200.1424322482435829No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT42510.14015728872302566No Hit
ACTTAGGAGGCTGGCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT40850.13468419770255466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40310.13290379459950985No Hit
CAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA39780.13115636192429925No Hit
CTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGA39710.1309255689294601No Hit
GTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACTATCACCAGCC39270.12947487010475695No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG38160.12581515261516488No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA35790.11800116121846833No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35590.11734175266178506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35290.11635263982676018No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34990.11536352699173531No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33880.11170380950214324No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT33140.10926399784241521No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32990.10876944142490276No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32980.1087364709970686No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32710.10784626944554619No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32620.10754953559503873No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG31580.10412061110028582No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31100.10253803056424601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88300.040.7802931
TATCAAC96700.037.0324972
TCAACGC100550.035.3039784
ATCAACG101200.035.1075743
CAACGCA101300.035.041445
AACGCAG103450.034.3131756
ACGCGAA800.003559078332.3502470
ACGCAGA111000.032.0398837
AGAGTAC115350.030.89301511
CGCAGAG115650.030.8098268
GTGGTAT13400.030.6682051
CAGAGTA117450.030.36629510
GCAGAGT117700.030.186959
AGTACGG92250.027.50615313
GAGTACG93900.027.0228212
TACGGGA32150.024.91681515
TACGGGG51250.024.82529615
GTACGGG102100.024.7206814
ACGGGGG15650.022.58917816
ACGGGGA26350.022.3605716
Produced by FastQC (version 0.11.5)