FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392880
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT138070.9912555281144104No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC26430.18975073229567516No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT22950.1647665269082764RNA PCR Primer, Index 17 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17030.12226466027224168No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT16970.12183389811038997RNA PCR Primer, Index 17 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG28650.057.91261770
CACGACG250.0063109253.79974469
ATCGCGT352.2636488E-549.3203437
AAGGGGG25900.045.87172769
TATACAC26150.039.2990763
CTTATAC27050.037.995621
AAGTACG1650.037.6912771
TTATACA27300.037.5264322
CCGTCTT27200.037.4773247
GCCGTCT27100.037.2159746
CGTATGC27950.036.8340341
AAAGGGG29700.036.53349768
TCGTATG27750.036.39984540
TATGCCG28500.036.17375643
ATGCCGT28350.036.1171244
TGCCGTC28050.036.0949345
TTCGCGA700.002171583735.7778270
AATCTCG26100.035.14620236
ATCTCGT26350.034.85178837
CTCGTAT27250.034.8128839
Produced by FastQC (version 0.11.5)