FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450065
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG364801.4889400893445683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78500.036.918711
GGTATCA33300.034.91171
TATCAAC85950.033.559142
ATCAACG89150.032.1942983
TCAACGC89700.031.997554
CAACGCA91050.031.5951145
AACGCAG92900.031.0371176
ACGCAGA99000.029.0579267
AGAGTAC103600.027.95978511
CGCAGAG103500.027.7295158
CAGAGTA104600.027.50112310
GTGGTAT13250.026.997031
GCAGAGT106700.026.9598669
TGGTATC13450.025.823032
AGTACGG76650.024.89212613
GAGTACG78400.024.37867512
GTACGGG84900.022.35734714
TACGGGG36000.021.9588215
TACGGGA26050.021.58513615
GAGTACT46750.020.72451212
Produced by FastQC (version 0.11.5)