FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392566
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG325981.3624702516043445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50780.21224074905352663No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC47260.1975285112302022No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38890.16254515026962682No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCAT37550.15694446882552038No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34630.1447399988129899No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33840.1414381045287779No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32110.13420737400765537No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCA31910.1333714514040574No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31580.13199217910812075No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30860.12898285773516802No Hit
GGATATTAGGAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATGGTGCATC29880.12488683697753791No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT28040.11719634902443653No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27480.1148557657343622No Hit
GGCCAAAAGTGTACGGGAAACTGTTAGCCTGTTGACAATAGTAAGTTGCA26290.10988202624295422No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26240.10967304559205472No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25350.10595319000604372No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGCTAACAGTTTC25040.10465750997046684No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24620.1029020725029111No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24480.10231692668039251No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24400.10198255763895334No Hit
GTGCAGCCACAGTTCGTTTCATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24370.10185716924841362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72600.037.6530721
TATCAAC80500.033.84062
TCAACGC82800.032.3294034
ATCAACG83250.032.31223
CAACGCA84200.031.793195
AACGCAG85700.031.5086
ACGCAGA91600.029.5148167
AGAGTAC93000.028.93067611
CAGAGTA95150.028.41363110
CGCAGAG95650.028.2651028
AGTACGG71200.027.5734613
GAGTACG73050.026.96554212
TACGGGG30850.025.85092215
GTCCGGT800.00117329925.19217968
GCCGTAT2500.024.60526354
CGTATCA2600.023.80691556
GTGGTAT13650.023.3885781
GTGGTCG2750.023.23394448
TACGGGT18100.022.21415
TTACGCT750.002487022222.1412284
Produced by FastQC (version 0.11.5)