FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386140
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT137020.5742328614414913No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56410.23640691661008995No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC53840.22563638344774406No Hit
CCTATGTACTGACTCAGCCACCCGCGGTGTCAGTGGCCCCAGGACAGACG52870.2215712405810221No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49550.20765755571760247No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47340.19839573537177196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT45930.19248661017375343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG45120.18909200633659384No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT44130.18494304609117654No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42910.1798301859907633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41620.17442396506491656No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG40750.17077790909167107No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38270.16038455413345404No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38260.16034264544410637No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACTACATCACTTGAACTATCCCACACCTGA36250.15191899888522886No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC35770.1499073817965417No Hit
GTGTCTGTGATCTCCTATGTACTGACTCAGCCACCCGCGGTGTCAGTGGCCCCAGGACAGACGGCCAGAATTAC34580.14492024776417142No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA34150.1431181741222225No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGGTTCGACACTTC33140.1388853964981099No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACTACATCACTTGAACTATCCCACACCTGACAGTAATAGT32670.13691568809877042No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31790.13322772343617725No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31740.133018179989439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31550.1322219148918337No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG31020.13000075435640826No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC30300.1269833287233775No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG30250.12677378527663927No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30240.1267318765872916No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30080.12606133755772922No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC29600.12404972046904206No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC29380.12312772930339376No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT29070.12182855993361663No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28740.12044557318514419No Hit
GAATTACCTGTGGGGGATTTAATATTGGAAGTAAAGAAGTTAACTGGTACCAGCAGAAGCCAGGCCAGGCCC28130.11788914313493759No Hit
CTACATCACTTGAACTATCCCACACCTGACAGTAATAGTCGGCCTCATCC27090.11353063944278206No Hit
GTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCA27020.11323727861734853No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG26920.11281819172387203No Hit
GTGTGGGATAGTTCAAGTGATGTAGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCC26810.11235719614104789No Hit
GGACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTTAACTTCTTTACTT26360.11047130512040367No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25780.10804060113824002No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC25770.10799869244889236No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA25610.10732815341932996No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24780.10384973220347507No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24530.10280201496978382No Hit
GTGATGTAGTGTTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCC23970.10045512836631548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG19300.088.49345470
AAGGGGG22200.051.67701369
TGGTATC11800.042.7974322
GTGGTAT12000.042.087671
GTATCAA101150.040.1406021
GGTATCA37750.038.4751551
CGTATGC26750.038.2987941
AAAGGGG27500.038.26902868
CTCGTAT25500.038.2241239
CTTATAC26700.038.2025071
ATGCCGT27100.038.12154844
TATACAC27250.038.0315553
TGCCGTC27000.038.0037445
TCGTATG26750.038.00136640
CGTATAC350.001660464137.79138624
TCTCGTA25650.037.7014138
CCGTCTT27650.037.35708647
TTATACA27800.036.9252852
ATCTCGT26400.036.87869637
AGATCCG750.001909489436.7295470
Produced by FastQC (version 0.11.5)