FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855395
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT188550.6603289562389791No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT71000.248652112930085No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC59370.20792219640364995No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49600.17370626480749599No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC49380.17293579347165627No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47400.16600155144909898No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46450.16267451613524575No Hit
GATATATGCTGCGGGCACTTTGCAGAGAGGAGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGAGAGATTTCA43510.15237821737447882No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43480.1522731531014098No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43360.1518528960091336No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC42490.14880603209013113No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40800.14288741137390798No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37930.13283626258363554No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG36540.1279682845981029No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36170.12667249189691793No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35460.12418597076761709No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35310.12366064940227185No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34440.1206137854832694No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33650.11784709295911774No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33500.1173217715937725No Hit
GAGGAGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGAGAGATTTCACT33420.11704160019892168No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT31950.11189345081853824RNA PCR Primer, Index 27 (95% over 23bp)
TCTTACTACTGTCAACAGAGTTTCTCTATTGTTCGGACGTTCGGCCAAGGGACCAAGGTAGAGATCAAACGAA31420.1100373153276517No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTTGGAGACACAATCACCATCACCTGCCGGGCCAGTCAGAGCATTAGTAG31140.10905671544567389No Hit
GCATATATCAGGAGTTTTGGGGGTCCCCCTGACTTTTGCTGATACCAATT30670.10741070850092543No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29800.104363844581923No Hit
GTAGTAAGATGCAACATCTTCAGGTTGTAGACTACTGATGGTGAGAGTGAAATCTCTCCCAGACCCACTGCCA29290.10257775193974915No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28590.10012625223480465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG31950.049.3947569
CTTATAC34550.038.032921
TATACAC35200.037.6933673
AAAGGGG39550.036.66977368
TCGTATG37550.036.14648440
TTATACA36500.035.9083632
AGGGGGG63200.035.89934570
CGTATGC37950.035.6959541
TGCCGTC38600.035.6161245
ATGCCGT39100.035.35661344
GTATCAA70450.035.1856081
CCGTCTT39600.034.97986647
GCCGTCT39650.034.6699546
TATCTCG37450.034.63530736
ATCTCGT37800.034.3916437
CTCGTAT39300.033.62466439
TATGCCG41150.033.34699643
GACGGAC39550.032.94368726
CTTGAAA45500.032.8357557
CGAGACG39600.032.83521323
Produced by FastQC (version 0.11.5)