FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855395
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384871.3478695592028422No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69300.24269847078950546No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52880.18519329199637877No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49510.17339107198828882No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49290.17262060065244914No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44720.1566158097215972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43830.15349890295388205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT41210.14432328977251832No Hit
GATATATGCTGCGGGCACTTTGCAGAGAGGAGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGAGAGATTTCA40860.1430975399200461No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39940.1398755688792619No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39710.13907007611906583No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39530.13843969048065152No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37440.13112021279017438No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34620.12124417112168367No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34520.12089395687812018No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33270.11651627883357644No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33190.11623610743872563No Hit
GAGGAGTCCCGTCAAGGTTCAGTGGCAGTGGGTCTGGGAGAGATTTCACT33170.11616606459001295No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32030.11217362221338904No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31690.11098289378527315No Hit
TCTTACTACTGTCAACAGAGTTTCTCTATTGTTCGGACGTTCGGCCAAGGGACCAAGGTAGAGATCAAACGAA31260.10947697253795008No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29800.104363844581923No Hit
GTAGTAAGATGCAACATCTTCAGGTTGTAGACTACTGATGGTGAGAGTGAAATCTCTCCCAGACCCACTGCCA29770.10425878030885394No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29580.10359337324608328No Hit
GTGCAGCCACAGTTCGTTTGATCTCTACCTTGGTCCCTTGGCCGAACGTC29160.10212247342311659No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTTGGAGACACAATCACCATCACCTGCCGGGCCAGTCAGAGCATTAGTAG29090.10187732345262215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73300.035.9056631
TATCAAC80200.032.503282
ATCAACG82300.031.3846073
TCAACGC83000.031.119374
CAACGCA83650.030.839855
AACGCAG85700.030.2157346
AGAGTAC90600.028.58455311
GTGGTAT12750.028.2875061
CGCAGAG92700.028.004088
GCAGAGT96450.026.8816329
AGTACGG79550.026.15108913
GAGTACG80750.025.76246512
GACAGGT10600.024.5225721
CAGAGTA107600.024.27655410
TACGTAT1751.5737257E-424.04473370
GTACGGG88600.023.36959814
TACGGGA30300.023.3589415
ACGCAGA113800.022.983257
TACGGGG39800.022.9225315
ACGGGGA17150.022.15672916
Produced by FastQC (version 0.11.5)