EGAF00005578383 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005578383
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2385939
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	25502	1.0688454315051643	No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCACAA	8421	0.35294280365088965	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	6676	0.27980597995170875	No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA	5837	0.244641627468263	No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT	5346	0.22406272750476858	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	5344	0.2239789030649987	No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA	4595	0.19258665037119557	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	4564	0.19128737155476314	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	4525	0.18965279497925136	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	4435	0.18588069518960879	No Hit
GCTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT	4249	0.17808502229101414	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	4167	0.1746482202604509	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	4038	0.16924154389529655	RNA PCR Primer, Index 26 (100% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	4025	0.16869668503679264	No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG	3812	0.15976938220130524	No Hit
ATATTATTCCTCCACCGCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT	3566	0.14945897610961553	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	3533	0.14807587285341328	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	3490	0.14627364739836182	No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT	3481	0.14589643741939756	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	3407	0.14279493314791367	No Hit
GTTTATTACTGTCAGCAATATTATTCCTCCACCGCTTTTGGCCAGGGGAC	3390	0.1420824254098701	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	3366	0.14107653213263205	No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT	3196	0.13395145475219608	RNA PCR Primer, Index 26 (100% over 24bp)
GTCCTGGTTTGTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTG	3156	0.1322749659567994	No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA	3114	0.13051465272163287	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	3090	0.12950875944439483	No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG	3085	0.12929919834497025	No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT	3077	0.1289639005858909	No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC	3037	0.12728741179049424	No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT	3005	0.12594622075417689	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	2952	0.12372487310027624	No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC	2875	0.1204976321691376	No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC	2763	0.11580346354202685	No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC	2724	0.11416888696651507	No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT	2692	0.11282769593019772	No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA	2615	0.10960045499905907	No Hit
GAATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC	2614	0.10955854277917415	No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGCG	2584	0.10830117618262664	No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA	2511	0.10524158413102766	No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG	2509	0.10515775969125783	No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA	2507	0.10507393525148799	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	7900	51.609047	70
AAGGGGG	5445	51.57796	69
GCGTATG	320	42.577274	40
CTTATAC	6065	42.28217	1
TATACAC	6245	41.570255	3
AAAGGGG	6275	40.78025	68
CGCGTAT	335	40.38228	39
GCCGTCT	5060	39.150307	46
TCGCGTA	335	39.04408	38
TGCCGTC	5185	38.705536	45
ATGCCGT	5210	38.63471	44
CGTATGC	5220	38.507248	41
CCGTCTT	5210	38.36803	47
ATCGCGT	465	38.262012	37
TCGTATG	5005	37.971523	40
CTTGAAA	5725	37.94014	57
CTCGTAT	4935	37.885468	39
TATGCCG	5495	37.258663	43
GTATGCC	5540	37.1682	42
ATCTCGT	5045	37.165234	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content