FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683154
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610731.6581712304182774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT108520.2946387796980523No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC89670.2434598173196125No Hit
GTCATAGGATTAGTTCCTATTTGAATTGGTATCAACAAAAACCAGGGAAAGCCCCTGAACTCCTGATCTATGCTG82740.2246444216017033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC81700.2218207547118584No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC81540.22138634442111302No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA72430.19665210849179807No Hit
GAGTTACGGTGCCCCTCCGACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCAT71510.19415424932001216No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT68570.18617196022756582No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG68010.184651524209957No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG63950.17362836308229304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC63280.1718092699897968No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT61950.16819823444797583No Hit
GTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCCCTTGGCCGAAGGTC61450.16684070228939654No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT60990.16559177270350356No Hit
TCCTATGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA58490.15880411191060706No Hit
CCGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGAC56620.15372694163752046No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC56080.1522608069062548No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATAGGATTAGTTCCTATTTGAATTGGTATCAACAAAAA55060.14949144130275302No Hit
GATCTATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTAGGTCAGATTTCA53880.14628766540850585No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT53710.1458261044745889No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGACCTAGATCCACTGCCA52290.1419707131442237No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG50890.13816962310020164No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTT50590.13735510380505403No Hit
ACTTACTACTGTCAACAGAGTTACGGTGCCCCTCCGACCTTCGGCCAAGGGACACGAGTGGAGATTAAACGAA48590.1319249751707368No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT48160.1307574975143586No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT47920.13010588207824056No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47760.1296714717874952No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG47050.12774377612231255No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG45610.12383408350560415No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC45530.12361687836023147No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCATAGGATTAGTTC44640.12120047111796031No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCACTCGTGTCC44400.12054885568184226No Hit
GATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGTTGATACCAATTCAAAT44340.12038595182281274No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG43230.11737223043076668No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT43090.11699212142636448No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42390.11509157640435344No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG41860.11365259231625939No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA41520.11272947044842546No Hit
GCATAGATCAGGAGTTCAGGGGCTTTCCCTGGTTTTTGTTGATACCAATT41010.11134478764667456No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40930.11112758250130188No Hit
GGACACGAGTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC40790.11074747349689967No Hit
GTTCAGTGGCAGTGGATCTAGGTCAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA40140.10898268169074657No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39020.10594180965552893No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA39000.10588750836918576No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT38600.10480148264232231No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC38570.10472003071280757No Hit
ATGCTGCATCCACTTTGCAGACTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTAGGTCAGATTTCACTCTCA37620.10214071961150689No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA37300.10127189903001613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT17050.038.7497521
GCCGTAT2550.034.95745554
CGCCGTA2750.033.58643753
CCGTATC2800.032.02159555
ATCAACG107850.029.4996323
TCAACGC108650.029.2824234
CAACGCA109500.029.0551195
AACGCAG114200.027.9739176
CGCAGAG118050.026.9782738
AGAGTAC118600.026.88202511
ACGAATA3100.026.30505224
ACGGGAT4100.026.17276216
GCAGAGT122300.026.1201239
CGTATCA3350.025.7904356
GAGTACG106800.024.92343912
AGTACGG107000.024.81610513
CGAATAG3500.023.33150925
CGATTGC1352.1852647E-623.31563463
TCGCTAG700.001873705723.2471221
GTACGGG114750.023.05541414
Produced by FastQC (version 0.11.5)