FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255472
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250411.9945486637694825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15430.12290198427364368No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13610.1084054443269145No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13340.10625485873042172No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12930.10298915467648821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1104.456524E-1034.00494456
GTATCAA56800.033.0066341
GGTATCA26750.032.3656961
GCCGTAT1157.894414E-1032.232354
GTGGTAT10700.031.9398291
TATCAAC62700.029.6505012
TGGTATC11050.029.4131362
ATCAACG64900.028.2380163
TCAACGC65250.028.0356314
CAACGCA65950.027.738065
AACGCAG67050.027.281916
AGAGTAC69200.026.15152411
CAGAGTA71900.025.3933910
CCGACGA1301.1597876E-825.06308621
GCAGAGT72900.025.0470549
CGCAGAG73000.025.0127438
TACGGGG20200.023.97484415
GACAGGT4250.023.7410451
GAGTACG59450.023.71936412
ACGGGAC2900.023.54683716
Produced by FastQC (version 0.11.5)