FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196048
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG414431.2966951685331385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57870.18106736819972666No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC56160.17571701050797736No Hit
GGCTAACAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCAT41080.1285337391678723No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40230.1258742046427338No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38960.12190054717576206No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37120.11614343714487392No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36670.1147354482786241No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATGAAACGAACTGTGGCTGCACCA36180.11320230484648541No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33620.10519241262959755No Hit
GGATATTAGGAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCCATGGTGCATC32370.10128133244557028No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32220.10081200282348701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11350.039.137761
GTATCAA80600.038.743861
TATCAAC88700.035.212292
TCAACGC94500.033.042324
ATCAACG94600.032.935843
CAACGCA95650.032.714295
AACGCAG98800.031.8388466
AGAGTAC103700.030.01650411
CGCAGAG105750.029.8089898
CAGAGTA106850.029.28518310
ACGCAGA112200.028.095377
CGTATCA2000.027.45589856
AGTACGG90800.027.3522613
GAGTACG93650.026.62594412
TGCGTAA550.00922218326.62390156
ACGCGTT500.00944979626.49116311
GCCGTAT2450.025.21424554
TTCGACG750.001411821624.40524356
TACGGGG47350.023.43353515
ACGGGGC13150.023.17697116
Produced by FastQC (version 0.11.5)