FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645437
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311141.176138384697878No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48070.18170910892982897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36610.13838923399045222No Hit
GTGCACACTGGCCTCTCGGTTTCGGCGGAGGGACCAAGGTGGAGATCAAA32950.12455409068520627No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32910.12440288693323635No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32300.12209702971569536No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31280.11824133404046287No Hit
CGTATACACTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG30720.11612448151288425No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27890.10542681606101373No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27160.10266734758756305No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAAATCTAGTCAAAGCCTCGTA26880.10160892132377373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71550.037.5590671
GCCGTAT1650.035.07179354
GGTATCA30600.033.518161
TATCAAC81700.032.9068952
ATCAACG83900.031.6384643
TCAACGC84250.031.470524
CAACGCA85600.031.0124745
AACGCAG85850.031.000256
GTGGTAT11500.028.9569641
AGAGTAC93700.028.54728711
CGCAGAG96150.027.6783228
CAGAGTA97950.027.34001410
ACGCAGA97900.027.1500977
GCAGAGT98450.027.0660029
ACGCGTT500.00923586126.61572611
AGTACGG79000.026.32242613
GAGTACG80200.025.80411712
TGGTATC13000.024.5711522
CGTATCA2250.024.27451756
ACGGGAC4950.024.21030616
Produced by FastQC (version 0.11.5)