EGAF00005578393 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005578393
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2012722
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	43937	2.1829641649467737	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	6301	0.3130586340289419	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	6190	0.3075437144324949	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	4548	0.22596265157334197	RNA PCR Primer, Index 3 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4029	0.2001766761629276	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	2150	0.10682051470595542	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	2076	0.10314390164165742	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	8400	0.0	75.87511	70
AAGGGGG	7710	0.0	59.18191	69
AAAGGGG	8770	0.0	47.498325	68
CTTATAC	8255	0.0	44.873203	1
CGCGTAT	85	1.8189894E-12	44.836697	39
TTATACA	8645	0.0	43.182625	2
GCCGTCT	8905	0.0	41.591774	46
CCGTCTT	9040	0.0	41.446255	47
TGCCGTC	9050	0.0	41.213116	45
ATGCCGT	9105	0.0	41.07162	44
CCGTCGT	150	0.0	41.02712	47
CTTGAAA	9525	0.0	40.857567	57
GCTTGAA	9485	0.0	40.806664	56
TATACAC	9500	0.0	40.78959	3
CGTATGC	9070	0.0	40.63721	41
TCGTATG	9010	0.0	40.26526	40
TGCTTGA	9510	0.0	40.1076	55
TATGCCG	9320	0.0	40.08131	43
CGTCTTC	9335	0.0	39.754257	48
ATCTCGT	8625	0.0	39.312313	37