EGAF00005578395 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005578395
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1566262
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	30725	1.961676909737962	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	4751	0.3033336695904006	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	4552	0.2906282601506006	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTCT	3430	0.21899273557042181	RNA PCR Primer, Index 1 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2618	0.16714955735375053	No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	1875	0.11971177235992445	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	1715	0.10949636778521091	No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA	1699	0.10847482732773955	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	1674	0.10687867036294056	No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC	1637	0.10451635805503806	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1568	0.10011096483219283	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	5595	68.86435	70
AAGGGGG	4645	59.94608	69
AAAGGGG	5335	47.648994	68
CTTATAC	5565	40.81592	1
TATACAC	5790	39.6207	3
TTATACA	5810	39.14953	2
CCGTCTT	6000	38.411934	47
GCCGTCT	6045	38.0652	46
GCTTGAA	6275	37.773983	56
TGCTTGA	6235	37.75846	55
CTTGAAA	6350	37.705414	57
TGCCGTC	6140	37.443047	45
TCGTATG	6020	37.060528	40
CGTATGC	6145	37.047867	41
ATGCCGT	6215	37.040398	44
TATGCCG	6205	36.991524	43
GTATCAA	4170	36.91173	1
ATCTCGT	5795	36.15102	37
TACACAT	6275	36.14354	5
CTGCTTG	6315	36.005318	54