FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271214
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG199460.8782087465117774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26470.11654560072278528No Hit
CTGTTAGCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC26040.1146523401141416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68650.036.641231
GTGGTAT10050.033.660921
TATCAAC74400.033.420532
ATCAACG78050.031.7620753
TCAACGC78250.031.7250464
AACGCAG78950.031.6118766
CAACGCA78900.031.54785
ACGCAGA85300.029.2585897
AGAGTAC86600.028.66989511
CGCAGAG87200.028.4676118
CAGAGTA88900.028.1496510
AGTACGG74700.025.93627513
GAGTACG77000.025.37884112
GGTATCA44550.025.0361291
GCAGAGT106500.023.5914129
TACGGGG38100.023.54367415
ACGGGGG14050.021.92108316
TTAACGG1907.8034645E-1021.54173735
CCGTTAA1405.048878E-721.52112816
AATAGCG1809.6213626E-820.52827362
Produced by FastQC (version 0.11.5)