FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523129
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378291.4992891762569414No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA43960.1742281112063632No Hit
ATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT41340.16384417919178923No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38160.15124078079242084No Hit
GTATAATATTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTC36370.1441464150267386No Hit
GTATAAAACACTCTGACTGGACTTGCAGTGGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA30770.12195175117879425No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27990.11093368591142189No Hit
GTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA27650.10958615274922526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26960.10685145309653211No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25670.10173875374584496No Hit
ACTTAGGAGGCTGGCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT25580.10138205379114583No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25410.10070828721004753No Hit
GTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAA25280.10019305394214882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118300.034.9129681
TATCAAC127050.032.237762
ATCAACG131000.030.7006443
TCAACGC130400.030.6616124
CAACGCA131550.030.4939635
AACGCAG131900.030.4368726
CGTATCA2600.029.60002956
ACGCAGA140650.028.5891727
AGAGTAC140650.028.56909811
CAGAGTA143600.028.02373910
GTGGTAT24700.027.846811
GCAGAGT144700.027.6287369
AGTACGG107350.026.2685413
CGCAGAG152650.026.1893148
GAGTACG110050.025.80357712
TACGGGG44100.025.01794215
GTACGGG115300.024.20004314
ACGGGGC9850.024.15241616
TACGGGA29350.023.97260515
GCCGACG3050.023.87751220
Produced by FastQC (version 0.11.5)