FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005578401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173588
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT186890.5888918158248645No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA79440.25031604606521074No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77030.24272211767879132No Hit
ATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT63170.19904915193780667No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62260.19618173499521677No Hit
GTATAAAACACTCTGACTGGACTTGCAGTGGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA60660.19114012278846532No Hit
GTATAATATTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTC57400.18086783791720917No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53140.16744454541673337No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52960.1668773640434738No Hit
ACTTAGGAGGCTGGCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT47200.14872756009916852No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47140.14853849964141533No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46460.14639581445354596No Hit
GTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACTATCACCAGCC44840.1412911820942101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT44710.14088155110241152No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG44050.13880188606712654No Hit
CACTATCACCAGCCTGCAGGCTGAAGATGTGGCAGTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTT42950.1353357776749849No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42050.13249987080868722No Hit
GTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAA41540.13089285691778518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40910.12890772211137677No Hit
GTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA40230.1267650369235074No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT38100.12005339067326949No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37590.11844637678236747No Hit
CTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGA37410.11787919540910792No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT37180.1171544636543874No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36970.11649275205225128No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36880.11620916136562148No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36520.11507479861910241No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36420.11475969785618045No Hit
CAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA36080.11368835526224576No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC35740.11261701266831108No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34630.10911939419987725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)