FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005578402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005578402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173588
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG541091.7049787180944722No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74880.23594745127596906No Hit
GTTTTATACAGTTCCAACAATAAGAACTACTTAGCTTGGTATCAGCAGAA69500.2189950302307672No Hit
ATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT67110.21146412199693218No Hit
GTATAATATTGCTGACAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTC63730.2008137162101697No Hit
GTATAAAACACTCTGACTGGACTTGCAGTGGATGGCGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA57840.18225428127406582No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC56190.17705511868585336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52380.1650497796185264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50750.15991363718289836No Hit
GTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA47790.15058665460040813No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA44960.14166930300971645No Hit
ACTTAGGAGGCTGGCCTGGTTTCTGCTGATACCAAGCTAAGTAGTTCTTATTGTTGGAACTGTATAAAACACT43740.13782507370206845No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT43610.1374154427102699No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43010.13552483813273808No Hit
CAGTAATAGACTGCCACATCTTCAGCCTGCAGGCTGGTGATAGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA42660.1344219854625112No Hit
GTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGACCAAGGTGGAAATCAAACGAA42390.1335712134026219No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41310.13016812516306464No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG40030.12613483539766346No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39710.12512651295631316No Hit
CACTATCACCAGCCTGCAGGCTGAAGATGTGGCAGTCTATTACTGTCAGCAATATTATACTACTCCTCGGACGTT39620.1248429222696834No Hit
GTTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACTATCACCAGCC38360.12087265265686661No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC37540.11828882640090649No Hit
CTATTACTGTCAGCAATATTATACTACTCCTCGGACGTTCGGCCCAGGGA36720.11570500014494636No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT36400.11469667770359607No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT36000.11343627465190818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34640.10915090427616944No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33510.1055902656551512No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32960.10385721145908039No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA32640.1028488890177301No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGTGAC32150.10130489527941246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85000.037.2314721
GTGGTAT12450.033.1780361
TATCAAC96200.032.8963782
ATCAACG98600.031.8210013
TCAACGC98450.031.7730164
CAACGCA99000.031.5969965
AACGCAG101900.030.8252776
ACGCAGA107350.029.2291457
AAACGCG500.005970619629.10434250
AGAGTAC110600.028.46283511
CGTATCA3050.028.2568556
CGCAGAG111550.028.1568588
CAGAGTA113400.027.86936810
GCAGAGT113950.027.5638249
AGTACGG94200.026.10890413
GAGTACG97000.025.45572912
TACGGGG51250.023.77808815
GACAGGT12400.023.60681
GTACGGG104000.023.46157314
GCCGTAT3650.023.3916154
Produced by FastQC (version 0.11.5)